Detailed information of TA system TA147302

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 602578..603231
• Replicon: chromosome
• Accession: NZ_CP047691
• Organism: Serratia marcescens strain C110

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GV213_RS02815
• Protein ID: WP_047569495.1
• Coordinates: 602578..602928 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A5C7DBM3
• Locus tag: GV213_RS02820
• Protein ID: WP_019453270.1
• Coordinates: 602932..603231 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GV213_RS02800	598512..599309	+	798	WP_094859831.1	hypothetical protein	-
GV213_RS02805	599314..601506	+	2193	WP_160208040.1	hypothetical protein	-
GV213_RS02810	601523..602218	+	696	WP_094859829.1	hypothetical protein	-
GV213_RS02815	602578..602928	+	351	WP_047569495.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GV213_RS02820	602932..603231	+	300	WP_019453270.1	XRE family transcriptional regulator	Antitoxin
GV213_RS02825	603221..604138	-	918	WP_033636930.1	LysR family transcriptional regulator	-
GV213_RS02830	604307..605005	+	699	WP_033648567.1	CoA transferase subunit A	-
GV213_RS02835	605017..605670	+	654	WP_041037752.1	CoA transferase subunit B	-
GV213_RS02840	605684..606868	+	1185	WP_047569491.1	acetyl-CoA C-acetyltransferase	-
GV213_RS02845	606887..607810	+	924	WP_047569489.1	3-hydroxyacyl-CoA dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13560.84 Da
• Isoelectric Point: 8.2699

>T147302 WP_047569495.1 NZ_CP047691:602578-602928 [Serratia marcescens]
MWTIMTTEEFDRWLCEQDESTQEKVLAALVMLERAGPSLRRPFVDVLKGSLHPNMKELRIQHKGRPIRAFFAFDPARQAI
VLCAGDKTGNEKRFYKVMLPIADAQFTQYLMCYFKE

Nucleotide

• Length: 351 bp

>T147302 NZ_CP062901:856431-856838 [Escherichia coli O152:H23]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11013.71 Da
• Isoelectric Point: 7.3207

>AT147302 WP_019453270.1 NZ_CP047691:602932-603231 [Serratia marcescens]
MSTLKQLLASRTPESRRRIEEMAEEMLFEAHLHQLREALNISQQELAKTLGISQPSVAAIEGRGNELKISTLKRYVEAMG
GTLRLDVELPGGKHVGFTV

Nucleotide

• Length: 300 bp

>AT147302 NZ_CP062901:856184-856450 [Escherichia coli O152:H23]
ATGGACATTAATAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A5C7DBM3