Detailed information of TA system TA147258

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 602577..603230
• Replicon: chromosome
• Accession: NZ_CP047685
• Organism: Serratia marcescens strain 2838

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GV198_RS02820
• Protein ID: WP_047569495.1
• Coordinates: 602577..602927 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A5C7DBM3
• Locus tag: GV198_RS02825
• Protein ID: WP_019453270.1
• Coordinates: 602931..603230 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GV198_RS02805	598511..599308	+	798	WP_094859831.1	hypothetical protein	-
GV198_RS02810	599313..601505	+	2193	WP_160208040.1	hypothetical protein	-
GV198_RS02815	601522..602217	+	696	WP_094859829.1	hypothetical protein	-
GV198_RS02820	602577..602927	+	351	WP_047569495.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GV198_RS02825	602931..603230	+	300	WP_019453270.1	XRE family transcriptional regulator	Antitoxin
GV198_RS02830	603220..604137	-	918	WP_033636930.1	LysR family transcriptional regulator	-
GV198_RS02835	604306..605004	+	699	WP_033648567.1	CoA transferase subunit A	-
GV198_RS02840	605016..605669	+	654	WP_041037752.1	CoA transferase subunit B	-
GV198_RS02845	605683..606867	+	1185	WP_047569491.1	acetyl-CoA C-acetyltransferase	-
GV198_RS02850	606886..607809	+	924	WP_047569489.1	3-hydroxyacyl-CoA dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13560.84 Da
• Isoelectric Point: 8.2699

>T147258 WP_047569495.1 NZ_CP047685:602577-602927 [Serratia marcescens]
MWTIMTTEEFDRWLCEQDESTQEKVLAALVMLERAGPSLRRPFVDVLKGSLHPNMKELRIQHKGRPIRAFFAFDPARQAI
VLCAGDKTGNEKRFYKVMLPIADAQFTQYLMCYFKE

Nucleotide

• Length: 351 bp

>T147258 NZ_CP062889:c4449640-4449329 [Escherichia coli O100:H21]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11013.71 Da
• Isoelectric Point: 7.3207

>AT147258 WP_019453270.1 NZ_CP047685:602931-603230 [Serratia marcescens]
MSTLKQLLASRTPESRRRIEEMAEEMLFEAHLHQLREALNISQQELAKTLGISQPSVAAIEGRGNELKISTLKRYVEAMG
GTLRLDVELPGGKHVGFTV

Nucleotide

• Length: 300 bp

>AT147258 NZ_CP062889:c4449328-4449038 [Escherichia coli O100:H21]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A5C7DBM3