Detailed information of TA system TA147161

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 45430..45856
• Replicon: plasmid pLD26-1-135kb
• Accession: NZ_CP047667
• Organism: Escherichia coli strain LD26-1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: GUU86_RS24475
• Protein ID: WP_001312861.1
• Coordinates: 45430..45588 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	45632..45856 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUU86_RS24440	40476..40703	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
GUU86_RS24445	40797..41483	-	687	WP_000332484.1	PAS domain-containing protein	-
GUU86_RS24450	41674..42057	-	384	WP_000124981.1	relaxosome protein TraM	-
GUU86_RS24455	42334..42981	+	648	WP_000614936.1	transglycosylase SLT domain-containing protein	-
GUU86_RS24460	43278..44099	-	822	WP_001234469.1	DUF945 domain-containing protein	-
GUU86_RS24465	44221..44508	-	288	WP_000107535.1	hypothetical protein	-
GUU86_RS25110	44533..44739	-	207	WP_000547939.1	hypothetical protein	-
GUU86_RS24475	45430..45588	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	45632..45856	-	225	NuclAT_0	-	Antitoxin
-	45632..45856	-	225	NuclAT_0	-	Antitoxin
-	45632..45856	-	225	NuclAT_0	-	Antitoxin
-	45632..45856	-	225	NuclAT_0	-	Antitoxin
GUU86_RS24480	45668..45856	+	189	WP_001299721.1	hypothetical protein	-
GUU86_RS24485	45868..46587	-	720	WP_001276228.1	plasmid SOS inhibition protein A	-
GUU86_RS24490	46584..47018	-	435	WP_000845895.1	conjugation system SOS inhibitor PsiB	-
GUU86_RS24495	47073..49031	-	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
GUU86_RS24500	49090..49323	-	234	WP_000006004.1	DUF905 domain-containing protein	-
GUU86_RS24505	49379..49906	-	528	WP_000290793.1	single-stranded DNA-binding protein	-
GUU86_RS24515	50379..50651	-	273	WP_072133095.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1C / dfrA14 / qnrS1 / tet(A) / floR	iroB / iroC / iroD / iroE / iroN	1..135123	135123

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T147161 WP_001312861.1 NZ_CP047667:c45588-45430 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T147161 NZ_CP062871:c1049411-1049160 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT147161 NZ_CP047667:c45856-45632 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin