Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4170094..4170635 Replicon chromosome
Accession NZ_CP047665
Organism Escherichia coli strain LD26-1

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag GUU86_RS19870 Protein ID WP_000615976.1
Coordinates 4170094..4170372 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag GUU86_RS19875 Protein ID WP_000729704.1
Coordinates 4170375..4170635 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUU86_RS19855 4167676..4168254 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
GUU86_RS19860 4168460..4169227 + 768 WP_000333380.1 class II glutamine amidotransferase -
GUU86_RS19865 4169198..4169938 - 741 WP_001225679.1 murein L,D-transpeptidase -
GUU86_RS19870 4170094..4170372 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
GUU86_RS19875 4170375..4170635 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
GUU86_RS19880 4170821..4171594 + 774 WP_000543895.1 C40 family peptidase -
GUU86_RS19885 4171651..4172007 - 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
GUU86_RS19890 4172000..4172278 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
GUU86_RS19895 4172383..4174095 - 1713 Protein_3881 flagellar biosynthesis protein FlhA -
GUU86_RS19900 4174067..4174852 + 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 4162374..4172278 9904


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T147157 WP_000615976.1 NZ_CP047665:c4170372-4170094 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T147157 NZ_CP062871:374499-374675 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT147157 WP_000729704.1 NZ_CP047665:c4170635-4170375 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT147157 NZ_CP062871:374721-375137 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References