Detailed information of TA system TA147153

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3341656..3342258
• Replicon: chromosome
• Accession: NZ_CP047665
• Organism: Escherichia coli strain LD26-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: GUU86_RS16070
• Protein ID: WP_000897305.1
• Coordinates: 3341656..3341967 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GUU86_RS16075
• Protein ID: WP_000356397.1
• Coordinates: 3341968..3342258 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUU86_RS16040	3336686..3337471	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
GUU86_RS16045	3337570..3338169	+	600	WP_001295269.1	glucose-1-phosphatase	-
GUU86_RS16050	3338163..3339035	+	873	WP_000920762.1	virulence factor BrkB family protein	-
GUU86_RS16055	3339032..3339469	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
GUU86_RS16060	3339514..3340455	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
GUU86_RS16065	3340519..3341427	-	909	WP_001162704.1	alpha/beta hydrolase	-
GUU86_RS16070	3341656..3341967	+	312	WP_000897305.1	hypothetical protein	Toxin
GUU86_RS16075	3341968..3342258	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
GUU86_RS16080	3342863..3343081	+	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
GUU86_RS16085	3343301..3343543	+	243	WP_001087409.1	hypothetical protein	-
GUU86_RS16090	3343873..3344802	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
GUU86_RS16095	3344799..3345434	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
GUU86_RS16100	3345431..3346333	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T147153 WP_000897305.1 NZ_CP047665:3341656-3341967 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T147153 NZ_CP062870:c130767-130462 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT147153 WP_000356397.1 NZ_CP047665:3341968-3342258 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT147153 NZ_CP062870:c130987-130769 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7