Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2538220..2539019 Replicon chromosome
Accession NZ_CP047665
Organism Escherichia coli strain LD26-1

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag GUU86_RS12195 Protein ID WP_000347266.1
Coordinates 2538555..2539019 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag GUU86_RS12190 Protein ID WP_001307405.1
Coordinates 2538220..2538555 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUU86_RS12175 2534005..2534775 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
GUU86_RS12180 2534791..2536125 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
GUU86_RS12185 2536500..2538071 + 1572 WP_001273741.1 galactarate dehydratase -
GUU86_RS12190 2538220..2538555 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
GUU86_RS12195 2538555..2539019 + 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
GUU86_RS12200 2539074..2539883 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
GUU86_RS12205 2540132..2541412 + 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
GUU86_RS12210 2541435..2541908 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
GUU86_RS12215 2541919..2542698 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
GUU86_RS12220 2542688..2543566 + 879 WP_001315856.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
GUU86_RS12225 2543584..2544018 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2527232..2539019 11787


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T147151 WP_000347266.1 NZ_CP047665:2538555-2539019 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T147151 NZ_CP062870:83881-84279 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT147151 WP_001307405.1 NZ_CP047665:2538220-2538555 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT147151 NZ_CP062870:83654-83881 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References