Detailed information of TA system TA147132

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 614985..615356
• Replicon: chromosome
• Accession: NZ_CP047665
• Organism: Escherichia coli strain LD26-1

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: GUU86_RS03055
• Protein ID: WP_001317028.1
• Coordinates: 615162..615356 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	614985..615163 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUU86_RS03030	610940..612313	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
GUU86_RS03035	612442..613377	-	936	WP_001153728.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
GUU86_RS03040	613429..614664	-	1236	WP_000040852.1	site-specific integrase	-
GUU86_RS03045	614666..614881	-	216	WP_000079604.1	excisionase XisR	-
GUU86_RS03050	614960..615169	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	614985..615163	+	179	NuclAT_0	-	Antitoxin
-	614985..615163	+	179	NuclAT_0	-	Antitoxin
-	614985..615163	+	179	NuclAT_0	-	Antitoxin
-	614985..615163	+	179	NuclAT_0	-	Antitoxin
GUU86_RS03055	615162..615356	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
GUU86_RS03060	615413..616222	-	810	WP_000166319.1	recombination protein RecT	-
GUU86_RS03065	616215..618815	-	2601	WP_001532611.1	exodeoxyribonuclease VIII	-
GUU86_RS03070	618917..619192	-	276	WP_000632297.1	protein RacC	-
GUU86_RS03075	619267..619437	-	171	WP_001352098.1	conserved protein, Rac prophage	-
GUU86_RS03080	619437..619658	-	222	WP_000560225.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T147132 WP_001317028.1 NZ_CP047665:c615356-615162 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T147132 NZ_CP062868:1327267-1327665 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT147132 NZ_CP047665:614985-615163 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin