Detailed information of TA system TA147126

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 48687..49398
• Replicon: plasmid pLD93-1-90kb
• Accession: NZ_CP047663
• Organism: Escherichia coli strain LD93-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UJZ0
• Locus tag: GUU84_RS22960
• Protein ID: WP_000162415.1
• Coordinates: 48687..48989 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GUU84_RS22965
• Protein ID: WP_000806445.1
• Coordinates: 49060..49398 (+)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUU84_RS22930	43706..44386	-	681	WP_000812238.1	hypothetical protein	-
GUU84_RS22935	44370..45275	-	906	WP_032342061.1	recombination-associated protein RdgC	-
GUU84_RS22940	45335..45988	-	654	WP_032342060.1	hypothetical protein	-
GUU84_RS22945	45985..46965	-	981	WP_000046500.1	hypothetical protein	-
GUU84_RS22950	46969..47736	-	768	WP_000203292.1	hypothetical protein	-
GUU84_RS22955	47733..48524	-	792	WP_109157910.1	phage tail protein	-
GUU84_RS22960	48687..48989	+	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GUU84_RS22965	49060..49398	+	339	WP_000806445.1	HigA family addiction module antidote protein	Antitoxin
GUU84_RS22975	49677..50405	+	729	WP_023908595.1	hypothetical protein	-
GUU84_RS22980	50439..51632	-	1194	WP_001112629.1	hypothetical protein	-
GUU84_RS22985	51625..51954	-	330	WP_000542383.1	hypothetical protein	-
GUU84_RS22990	52283..52936	-	654	WP_023908597.1	hypothetical protein	-
GUU84_RS22995	53205..54116	+	912	WP_080028516.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..90674	90674

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11818.56 Da
• Isoelectric Point: 9.8739

>T147126 WP_000162415.1 NZ_CP047663:48687-48989 [Escherichia coli]
MTKKINIKDFRDAWLDDFFEFSTPHRKIPPDIHMTLSRKLDIINAATTCKDLRSPPGNRYEELSGKLNGYSSVRVNKQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T147126 NZ_CP062867:132479-132895 [Escherichia coli O39:H21]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13049.98 Da
• Isoelectric Point: 6.4754

>AT147126 WP_000806445.1 NZ_CP047663:49060-49398 [Escherichia coli]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRINTAEKFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT147126 NZ_CP062867:132252-132482 [Escherichia coli O39:H21]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A631VBQ3