Detailed information of TA system TA147109

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 899581..900275
• Replicon: chromosome
• Accession: NZ_CP047662
• Organism: Escherichia coli strain LD93-1

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: GUU84_RS04145
• Protein ID: WP_001263493.1
• Coordinates: 899877..900275 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: GUU84_RS04140
• Protein ID: WP_000554757.1
• Coordinates: 899581..899874 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUU84_RS04115	895398..895865	+	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-
GUU84_RS04120	895885..896601	+	717	WP_000938723.1	FliA/WhiG family RNA polymerase sigma factor	-
GUU84_RS04125	896614..897477	+	864	WP_094318826.1	flagellar motor stator protein MotA	-
GUU84_RS04130	897480..898403	+	924	WP_001232547.1	putative lateral flagellar export/assembly protein LafU	-
GUU84_RS04135	898474..899529	+	1056	WP_094318827.1	DNA polymerase IV	-
GUU84_RS04140	899581..899874	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
GUU84_RS04145	899877..900275	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
GUU84_RS04150	900285..900737	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
GUU84_RS04155	900983..901186	+	204	Protein_804	RNA ligase RtcB family protein	-
GUU84_RS04160	901185..901706	+	522	Protein_805	peptide chain release factor H	-
GUU84_RS04165	901763..903220	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
GUU84_RS04170	903481..903939	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
GUU84_RS04175	904031..905275	+	1245	WP_000189541.1	esterase FrsA	-
-	904535..904615	+	81	NuclAT_10	-	-
-	904535..904615	+	81	NuclAT_10	-	-
-	904535..904615	+	81	NuclAT_10	-	-
-	904535..904615	+	81	NuclAT_10	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T147109 WP_001263493.1 NZ_CP047662:899877-900275 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T147109 NZ_CP062865:c2526044-2525941 [Escherichia coli O39:H21]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT147109 WP_000554757.1 NZ_CP047662:899581-899874 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT147109 NZ_CP062865:2525903-2526174 [Escherichia coli O39:H21]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6