Detailed information of TA system TA147101

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 74207..74461
• Replicon: plasmid pLD39-1-134kb
• Accession: NZ_CP047660
• Organism: Escherichia coli strain LD39-1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: GUU82_RS24225
• Protein ID: WP_001351576.1
• Coordinates: 74207..74413 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	74400..74461 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUU82_RS24805	69308..69511	-	204	Protein_56	IS3 family transposase	-
GUU82_RS24190	69821..70141	+	321	Protein_57	transposase family protein	-
GUU82_RS24195	70168..70920	-	753	Protein_58	DUF4158 domain-containing protein	-
GUU82_RS24200	71044..71319	-	276	WP_089629733.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GUU82_RS24205	71319..71603	-	285	WP_089629734.1	ribbon-helix-helix domain-containing protein	-
GUU82_RS24210	72511..73367	-	857	Protein_61	incFII family plasmid replication initiator RepA	-
GUU82_RS24215	73360..73434	-	75	WP_061357229.1	RepA leader peptide Tap	-
GUU82_RS24220	73666..73923	-	258	WP_000083845.1	replication regulatory protein RepA	-
GUU82_RS24225	74207..74413	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	74400..74461	+	62	NuclAT_1	-	Antitoxin
-	74400..74461	+	62	NuclAT_1	-	Antitoxin
-	74400..74461	+	62	NuclAT_1	-	Antitoxin
-	74400..74461	+	62	NuclAT_1	-	Antitoxin
GUU82_RS24230	74803..75015	-	213	WP_032153777.1	hypothetical protein	-
GUU82_RS24235	75146..75706	-	561	WP_021563950.1	fertility inhibition protein FinO	-
GUU82_RS24240	75761..76501	-	741	WP_089590496.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..134831	134831

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T147101 WP_001351576.1 NZ_CP047660:c74413-74207 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T147101 NZ_CP062865:c815994-815716 [Escherichia coli O39:H21]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT147101 NZ_CP047660:74400-74461 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin