Detailed information of TA system TA147096

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 4104858..4105270
• Replicon: chromosome
• Accession: NZ_CP047658
• Organism: Escherichia coli strain LD39-1

Toxin (Protein)

• Gene name: symE
• Uniprot ID: B1IS82
• Locus tag: GUU82_RS19880
• Protein ID: WP_000132625.1
• Coordinates: 4104929..4105270 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	4104858..4104934 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUU82_RS19870	4101476..4102945	+	1470	WP_061359977.1	type I restriction-modification system subunit M	-
GUU82_RS19875	4102945..4104708	+	1764	WP_096973077.1	restriction endonuclease subunit S	-
-	4104858..4104934	-	77	NuclAT_13	-	Antitoxin
-	4104858..4104934	-	77	NuclAT_13	-	Antitoxin
-	4104858..4104934	-	77	NuclAT_13	-	Antitoxin
-	4104858..4104934	-	77	NuclAT_13	-	Antitoxin
-	4104858..4104934	-	77	NuclAT_14	-	Antitoxin
-	4104858..4104934	-	77	NuclAT_14	-	Antitoxin
-	4104858..4104934	-	77	NuclAT_14	-	Antitoxin
-	4104858..4104934	-	77	NuclAT_14	-	Antitoxin
GUU82_RS19880	4104929..4105270	+	342	WP_000132625.1	endoribonuclease SymE	Toxin
GUU82_RS19885	4105317..4107410	-	2094	WP_000648226.1	DUF262 domain-containing protein	-
GUU82_RS19890	4107507..4107671	-	165	WP_000394276.1	DUF1127 domain-containing protein	-
GUU82_RS19895	4107848..4109260	+	1413	WP_000199353.1	PLP-dependent aminotransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12270.10 Da
• Isoelectric Point: 8.4952

>T147096 WP_000132625.1 NZ_CP047658:4104929-4105270 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVVVKVMEGCIVLTAQPAAAEESE
LMQSLRQVCKLSARKQKQVQDFIGVITGKQKVA

Nucleotide

• Length: 342 bp

>T147096 NZ_CP062863:c4276012-4275839 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Nucleotide

• Length: 77 bp

>AT147096 NZ_CP047658:c4104934-4104858 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: B1IS82

Antitoxin