Detailed information of TA system TA147094

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3741972..3742666
• Replicon: chromosome
• Accession: NZ_CP047658
• Organism: Escherichia coli strain LD39-1

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: GUU82_RS18175
• Protein ID: WP_001263493.1
• Coordinates: 3741972..3742370 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: GUU82_RS18180
• Protein ID: WP_000554757.1
• Coordinates: 3742373..3742666 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUU82_RS18145	3736972..3738216	-	1245	WP_000189541.1	esterase FrsA	-
-	3737632..3737712	-	81	NuclAT_11	-	-
-	3737632..3737712	-	81	NuclAT_11	-	-
-	3737632..3737712	-	81	NuclAT_11	-	-
-	3737632..3737712	-	81	NuclAT_11	-	-
GUU82_RS18150	3738308..3738766	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
GUU82_RS18155	3739027..3740484	+	1458	WP_001293013.1	cytosol nonspecific dipeptidase	-
GUU82_RS18160	3740541..3741062	-	522	Protein_3546	peptide chain release factor H	-
GUU82_RS18165	3741061..3741264	-	204	Protein_3547	RNA ligase RtcB family protein	-
GUU82_RS18170	3741510..3741962	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
GUU82_RS18175	3741972..3742370	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
GUU82_RS18180	3742373..3742666	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
GUU82_RS18185	3742718..3743773	-	1056	WP_001226164.1	DNA polymerase IV	-
GUU82_RS18190	3743844..3744767	-	924	WP_001232547.1	putative lateral flagellar export/assembly protein LafU	-
GUU82_RS18195	3744770..3745633	-	864	WP_001169518.1	flagellar motor stator protein MotA	-
GUU82_RS18200	3745646..3746362	-	717	WP_000938730.1	FliA/WhiG family RNA polymerase sigma factor	-
GUU82_RS18205	3746382..3746849	-	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T147094 WP_001263493.1 NZ_CP047658:c3742370-3741972 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T147094 NZ_CP062863:c4018627-4018340 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTGGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCG
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGTCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT147094 WP_000554757.1 NZ_CP047658:c3742666-3742373 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT147094 NZ_CP062863:c4018353-4018051 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAATTGAAGGCGCTGGCGAA
TAAATCCGACGAAGAGATTGATTACAGCGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6