Detailed information of TA system TA147073

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 668059..668858
• Replicon: chromosome
• Accession: NZ_CP047658
• Organism: Escherichia coli strain LD39-1

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: S1NYM6
• Locus tag: GUU82_RS03205
• Protein ID: WP_000347251.1
• Coordinates: 668059..668523 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: GUU82_RS03210
• Protein ID: WP_012304834.1
• Coordinates: 668523..668858 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUU82_RS03175	663060..663494	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
GUU82_RS03180	663512..664390	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
GUU82_RS03185	664380..665159	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
GUU82_RS03190	665170..665643	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
GUU82_RS03195	665666..666946	-	1281	WP_000681905.1	tagatose-bisphosphate aldolase subunit KbaZ	-
GUU82_RS03200	667195..668004	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
GUU82_RS03205	668059..668523	-	465	WP_000347251.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
GUU82_RS03210	668523..668858	-	336	WP_012304834.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
GUU82_RS03215	669007..670578	-	1572	WP_001273760.1	galactarate dehydratase	-
GUU82_RS03220	670953..672287	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
GUU82_RS03225	672303..673073	+	771	WP_001058236.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17777.14 Da
• Isoelectric Point: 9.4947

>T147073 WP_000347251.1 NZ_CP047658:c668523-668059 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T147073 NZ_CP062858:97280-97441 [Escherichia coli O20:H12]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTAATATTCACACTCCTGACCCG
GAATCGTCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAGGTTACGGCAAGTTTGGCTTACGAATCCAGCGGTAAGT
AG

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12344.95 Da
• Isoelectric Point: 4.6890

>AT147073 WP_012304834.1 NZ_CP047658:c668858-668523 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT147073 NZ_CP062858:97016-97227 [Escherichia coli O20:H12]
TCACACGGATTACCCGTGAACTGGTTGAACGACCAGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
AGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGC
AGAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Toxin

• AlphaFold DB: A0A829CJ20