Detailed information of TA system TA147011

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5854579..5855174
• Replicon: chromosome
• Accession: NZ_CP047643
• Organism: Pseudomonas aeruginosa CI27

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: GTW26_RS27745
• Protein ID: WP_003113526.1
• Coordinates: 5854896..5855174 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GTW26_RS27740
• Protein ID: WP_003133769.1
• Coordinates: 5854579..5854884 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTW26_RS27705 (GTW26_27635)	5849720..5850568	+	849	WP_003095007.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
GTW26_RS27715 (GTW26_27645)	5850735..5851676	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
GTW26_RS27720 (GTW26_27650)	5851793..5852407	+	615	WP_003095013.1	50S ribosomal protein L25/general stress protein Ctc	-
GTW26_RS27725 (GTW26_27655)	5852449..5853033	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
GTW26_RS27730 (GTW26_27660)	5853074..5854174	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
GTW26_RS27740 (GTW26_27670)	5854579..5854884	-	306	WP_003133769.1	HigA family addiction module antitoxin	Antitoxin
GTW26_RS27745 (GTW26_27675)	5854896..5855174	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GTW26_RS27750	5855227..5855355	-	129	Protein_5492	integrase	-
GTW26_RS27755 (GTW26_27680)	5855503..5857731	+	2229	WP_003141628.1	TonB-dependent receptor	-
GTW26_RS27760 (GTW26_27685)	5857801..5858448	-	648	WP_003095021.1	carbonate dehydratase	-
GTW26_RS27765 (GTW26_27690)	5858510..5859748	-	1239	WP_003141631.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T147011 WP_003113526.1 NZ_CP047643:c5855174-5854896 [Pseudomonas aeruginosa CI27]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T147011 NZ_CP062851:856864-857271 [Escherichia coli O9:H10]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT147011 WP_003133769.1 NZ_CP047643:c5854884-5854579 [Pseudomonas aeruginosa CI27]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT147011 NZ_CP062851:856617-856883 [Escherichia coli O9:H10]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: V6ALY3