Detailed information of TA system TA146998

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 27011..27429
• Replicon: plasmid unnamed4
• Accession: NZ_CP047637
• Organism: Klebsiella pneumoniae strain K2606

Toxin (Protein)

• Gene name: hok
• Uniprot ID: A0A168PPB2
• Locus tag: GUY22_RS28810
• Protein ID: WP_012881135.1
• Coordinates: 27268..27429 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	27011..27215 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUY22_RS28780	22711..23232	+	522	WP_012881138.1	single-stranded DNA-binding protein	-
GUY22_RS28785	23288..23521	+	234	WP_000005971.1	DUF905 domain-containing protein	-
GUY22_RS28790	23585..25543	+	1959	WP_012881137.1	ParB/RepB/Spo0J family partition protein	-
GUY22_RS28795	25598..26032	+	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
GUY22_RS28800	26029..26748	+	720	WP_012881136.1	plasmid SOS inhibition protein A	-
GUY22_RS28805	26745..27059	+	315	WP_000872086.1	hypothetical protein	-
-	27008..27215	+	208	NuclAT_0	-	-
-	27008..27215	+	208	NuclAT_0	-	-
-	27008..27215	+	208	NuclAT_0	-	-
-	27008..27215	+	208	NuclAT_0	-	-
-	27011..27215	-	205	-	-	Antitoxin
GUY22_RS28810	27268..27429	+	162	WP_012881135.1	type I toxin-antitoxin system Hok family toxin	Toxin
GUY22_RS29270	27667..27936	-	270	WP_001388591.1	hypothetical protein	-
GUY22_RS28820	28275..28598	+	324	WP_000533253.1	hypothetical protein	-
GUY22_RS28825	28657..28899	+	243	WP_000540588.1	hypothetical protein	-
GUY22_RS28835	29896..30192	+	297	WP_001272251.1	hypothetical protein	-
GUY22_RS28840	30302..31123	+	822	WP_001234437.1	DUF945 domain-containing protein	-
GUY22_RS28845	31419..32021	-	603	WP_000243715.1	transglycosylase SLT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-14	-	1..70729	70729

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6000.17 Da
• Isoelectric Point: 8.5137

>T146998 WP_012881135.1 NZ_CP047637:27268-27429 [Klebsiella pneumoniae]
MKLPGNALIWCVLIVCCTLLIFTHLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 162 bp

>T146998 NZ_CP062838:c4269300-4269127 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Nucleotide

• Length: 205 bp

>AT146998 NZ_CP047637:c27215-27011 [Klebsiella pneumoniae]
AGACATACAGGGGGCCTCGTGGGTTAATGAAAAATTAACTACGGGGCTTTCTGCCTTCTGCCACACAACACGGTAACAAA
CCACCTTCACGTCATGAGGCAGAAAGCCTCAAGCGCCGGGCACATCATAGCCCATATACCTGCGCACTGACCACACTCAC
TTTCCCTGAAAATAATCCGGTCGTTCAGCCAGTTCACGGGCAATC

Structures

Toxin

• AlphaFold DB: A0A168PPB2

Antitoxin