Detailed information of TA system TA146971

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 12317..12846
• Replicon: plasmid pLraf_19_5_1
• Accession: NZ_CP047617
• Organism: Lactococcus raffinolactis strain Lr_19_5

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: GU336_RS12685
• Protein ID: WP_167839261.1
• Coordinates: 12580..12846 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: GU336_RS12680
• Protein ID: WP_167839260.1
• Coordinates: 12317..12580 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GU336_RS12670	7725..10238	+	2514	WP_167839258.1	type IV secretory system conjugative DNA transfer family protein	-
GU336_RS12675	10324..12240	+	1917	WP_167839259.1	hypothetical protein	-
GU336_RS12680	12317..12580	+	264	WP_167839260.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GU336_RS12685	12580..12846	+	267	WP_167839261.1	Txe/YoeB family addiction module toxin	Toxin
GU336_RS12690	12869..13501	+	633	WP_167839262.1	hypothetical protein	-
GU336_RS12695	13577..16963	+	3387	WP_167839263.1	LPXTG cell wall anchor domain-containing protein	-
GU336_RS12700	17071..17367	+	297	WP_167839264.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	msr(D) / mef(A)	-	1..82612	82612

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (4-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (6-69)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10487.84 Da
• Isoelectric Point: 9.7990

>T146971 WP_167839261.1 NZ_CP047617:12580-12846 [Lactococcus raffinolactis]
MANYQVVIKNSAKNDLKKLKQSNLKSSFLDIVETLANDPYEQSQSFEKLQPKKEGRYSRRINHQHRVVYKVNEELKQVEI
YSAWSHYE

Nucleotide

• Length: 267 bp

>T146971 NZ_CP062833:56649-57029 [Escherichia coli]
ATGAGACATATATCACCGGAAGAACTTGTTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCGGAGTAG

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9855.86 Da
• Isoelectric Point: 4.1953

>AT146971 WP_167839260.1 NZ_CP047617:12317-12580 [Lactococcus raffinolactis]
MTELSLSPSNARKNFYQLLKDVNANHSQVEIVSDRTGNNAVLISLSDWHSIQETLFLEQVGVMDKVRERENDDTGFSAVS
ELDWDSL

Nucleotide

• Length: 264 bp

>AT146971 NZ_CP062833:56428-56649 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA