Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type I Classification (family/domain) hok-sok/-
Location 46639..46903 Replicon plasmid pNMBU_W06E18_Str1_1
Accession NZ_CP047610
Organism Escherichia coli strain NMBU_ W06E18

Toxin (Protein)


Gene name pndA Uniprot ID U9Z417
Locus tag GU332_RS25605 Protein ID WP_001387489.1
Coordinates 46639..46791 (-) Length 51 a.a.

Antitoxin (RNA)


Gene name pndB
Locus tag -
Coordinates 46843..46903 (+)

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GU332_RS25570 41731..42264 - 534 WP_001221666.1 lipocalin family protein -
GU332_RS25575 42358..43503 - 1146 WP_039023136.1 class C extended-spectrum beta-lactamase CMY-42 -
GU332_RS25580 43956..44653 - 698 Protein_57 IS1 family transposase -
GU332_RS25585 44878..45087 - 210 WP_000062603.1 HEAT repeat domain-containing protein -
GU332_RS25590 45479..45655 + 177 WP_001054897.1 hypothetical protein -
GU332_RS25595 45720..46016 - 297 WP_011264046.1 hypothetical protein -
GU332_RS25600 46316..46567 + 252 WP_001291964.1 hypothetical protein -
GU332_RS25605 46639..46791 - 153 WP_001387489.1 Hok/Gef family protein Toxin
- 46843..46903 + 61 NuclAT_0 - Antitoxin
- 46843..46903 + 61 NuclAT_0 - Antitoxin
- 46843..46903 + 61 NuclAT_0 - Antitoxin
- 46843..46903 + 61 NuclAT_0 - Antitoxin
GU332_RS25610 47106..47453 + 348 Protein_63 protein finQ -
GU332_RS25615 47639..48796 + 1158 Protein_64 IS1380-like element ISEc9 family transposase -
GU332_RS25620 48852..49549 + 698 Protein_65 IS1 family transposase -
GU332_RS25625 49563..49868 - 306 Protein_66 transcription termination factor NusG -
GU332_RS25630 50310..50597 - 288 WP_000074855.1 conjugal transfer protein TraA -
GU332_RS25635 50515..50778 - 264 WP_032180564.1 ash family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaCMY-42 - 1..51704 51704
- inside IScluster/Tn blaCMY-42 - 42358..49127 6769


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(7-46)


Sequences


Toxin        


Download         Length: 51 a.a.        Molecular weight: 5761.10 Da        Isoelectric Point: 8.7948

>T146964 WP_001387489.1 NZ_CP047610:c46791-46639 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Download         Length: 153 bp

>T146964 NZ_CP062832:2663810-2663917 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 61 bp

>AT146964 NZ_CP047610:46843-46903 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB U9Z417


Antitoxin

Download structure file

References