Detailed information of TA system TA146964

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 46639..46903
• Replicon: plasmid pNMBU_W06E18_Str1_1
• Accession: NZ_CP047610
• Organism: Escherichia coli strain NMBU_ W06E18

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: GU332_RS25605
• Protein ID: WP_001387489.1
• Coordinates: 46639..46791 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	46843..46903 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GU332_RS25570	41731..42264	-	534	WP_001221666.1	lipocalin family protein	-
GU332_RS25575	42358..43503	-	1146	WP_039023136.1	class C extended-spectrum beta-lactamase CMY-42	-
GU332_RS25580	43956..44653	-	698	Protein_57	IS1 family transposase	-
GU332_RS25585	44878..45087	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
GU332_RS25590	45479..45655	+	177	WP_001054897.1	hypothetical protein	-
GU332_RS25595	45720..46016	-	297	WP_011264046.1	hypothetical protein	-
GU332_RS25600	46316..46567	+	252	WP_001291964.1	hypothetical protein	-
GU332_RS25605	46639..46791	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
-	46843..46903	+	61	NuclAT_0	-	Antitoxin
-	46843..46903	+	61	NuclAT_0	-	Antitoxin
-	46843..46903	+	61	NuclAT_0	-	Antitoxin
-	46843..46903	+	61	NuclAT_0	-	Antitoxin
GU332_RS25610	47106..47453	+	348	Protein_63	protein finQ	-
GU332_RS25615	47639..48796	+	1158	Protein_64	IS1380-like element ISEc9 family transposase	-
GU332_RS25620	48852..49549	+	698	Protein_65	IS1 family transposase	-
GU332_RS25625	49563..49868	-	306	Protein_66	transcription termination factor NusG	-
GU332_RS25630	50310..50597	-	288	WP_000074855.1	conjugal transfer protein TraA	-
GU332_RS25635	50515..50778	-	264	WP_032180564.1	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCMY-42	-	1..51704	51704
	-	inside	IScluster/Tn	blaCMY-42	-	42358..49127	6769

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T146964 WP_001387489.1 NZ_CP047610:c46791-46639 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T146964 NZ_CP062832:2663810-2663917 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT146964 NZ_CP047610:46843-46903 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin