Detailed information of TA system TA146956

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4484701..4485303
• Replicon: chromosome
• Accession: NZ_CP047609
• Organism: Escherichia coli strain NMBU_ W06E18

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: GU332_RS21615
• Protein ID: WP_000897305.1
• Coordinates: 4484701..4485012 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GU332_RS21620
• Protein ID: WP_000356395.1
• Coordinates: 4485013..4485303 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GU332_RS21595	4479742..4480179	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
GU332_RS21600	4480224..4481165	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
GU332_RS21605	4481581..4483395	+	1815	WP_020233236.1	hypothetical protein	-
GU332_RS21610	4483564..4484472	-	909	WP_001306651.1	alpha/beta hydrolase	-
GU332_RS21615	4484701..4485012	+	312	WP_000897305.1	hypothetical protein	Toxin
GU332_RS21620	4485013..4485303	+	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
GU332_RS21625	4485388..4485600	+	213	WP_000197769.1	hypothetical protein	-
GU332_RS21630	4485662..4485940	+	279	WP_001306650.1	hypothetical protein	-
GU332_RS21635	4486359..4486577	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
GU332_RS21640	4486796..4487038	+	243	WP_021523315.1	hypothetical protein	-
GU332_RS21645	4487220..4488149	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
GU332_RS21650	4488146..4488781	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
GU332_RS21655	4488778..4489680	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T146956 WP_000897305.1 NZ_CP047609:4484701-4485012 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T146956 NZ_CP062832:c792079-791702 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT146956 WP_000356395.1 NZ_CP047609:4485013-4485303 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT146956 NZ_CP062832:c792537-792169 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAATTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7