Detailed information of TA system TA146950

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3101600..3102327
• Replicon: chromosome
• Accession: NZ_CP047609
• Organism: Escherichia coli strain NMBU_ W06E18

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A829L2T9
• Locus tag: GU332_RS15020
• Protein ID: WP_001521139.1
• Coordinates: 3102016..3102327 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GU332_RS15015
• Protein ID: WP_000126294.1
• Coordinates: 3101600..3102019 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GU332_RS14995	3096685..3097212	-	528	WP_001078777.1	electron transport protein HydN	-
GU332_RS15000	3097361..3098371	-	1011	WP_015912547.1	DNA-binding transcriptional regulator AscG	-
GU332_RS15005	3098631..3100088	+	1458	WP_020234027.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
GU332_RS15010	3100097..3101521	+	1425	WP_020234028.1	6-phospho-beta-glucosidase AscB	-
GU332_RS15015	3101600..3102019	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
GU332_RS15020	3102016..3102327	-	312	WP_001521139.1	type II toxin-antitoxin system HigB family toxin	Toxin
GU332_RS15025	3102494..3102964	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
GU332_RS15030	3102957..3103367	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
GU332_RS15035	3103364..3104131	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
GU332_RS15040	3104131..3104673	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
GU332_RS15045	3104683..3106392	-	1710	WP_001521140.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3079267..3131937	52670

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12493.23 Da
• Isoelectric Point: 9.4783

>T146950 WP_001521139.1 NZ_CP047609:c3102327-3102016 [Escherichia coli]
MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T146950 NZ_CP062831:137349-137606 [Escherichia coli]
GTGTTTAGTCACCGCATTGTGGTCTCGGCCATTACTTACTCTGAAATGCGCTTCGGTGCCTCCGGTCCGAAGGCCTCCCC
GCGTCACGTGCAACTGGTTGATAAATTCTGCCCGTGCCTCGATGCCATCCTGTCTTGGGACCGCGCCGCGGTGGACGGCA
CCACGGATATTAAAGTGGCACTGCGCCAGGCCGGGACGCCGATCGGCCAGAACGATACGGCGATTGCCGGGCCTGAAGCT
GGACGTATAGGAAATTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT146950 WP_000126294.1 NZ_CP047609:c3102019-3101600 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT146950 NZ_CP062831:137030-137260 [Escherichia coli]
ATGAGAACTGTATCCATTGTTAAAAACGGCAATAACCGCGCCATCCATCTGCCCCGCGATTTGGATTTTGAGCGGGTGAG
AGAGCTGGAGATCGTCCGCGAAGGAGACAGCATCATCCTGCGTCCCGTCCAGCCGAACTGGGGCTCATTCGCGCAGCTCG
AAAAAGCCGATCCGGATTTTATGGCGGAGCGCGAGAATGTTGTCAGCGACGAAGGTCGATTTGACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A829L2T9