Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2165419..2165921 Replicon chromosome
Accession NZ_CP047609
Organism Escherichia coli strain NMBU_ W06E18

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag GU332_RS10525 Protein ID WP_000767829.1
Coordinates 2165419..2165673 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag GU332_RS10530 Protein ID WP_001259255.1
Coordinates 2165670..2165921 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GU332_RS10495 2160434..2160661 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
GU332_RS10500 2160675..2161733 - 1059 WP_000492324.1 transport protein YeeE -
GU332_RS10505 2161912..2163270 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
GU332_RS10510 2163260..2163322 - 63 WP_010723108.1 membrane protein YoeI -
GU332_RS10515 2163537..2164466 - 930 WP_023154044.1 LysR family transcriptional regulator -
GU332_RS10520 2164512..2165336 - 825 WP_000754758.1 SDR family oxidoreductase -
GU332_RS10525 2165419..2165673 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
GU332_RS10530 2165670..2165921 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
GU332_RS10535 2166204..2166254 + 51 WP_001364200.1 his operon leader peptide -
GU332_RS10540 2166400..2167299 + 900 WP_021523143.1 ATP phosphoribosyltransferase -
GU332_RS10545 2167305..2168609 + 1305 WP_021523144.1 histidinol dehydrogenase -
GU332_RS10550 2168606..2169676 + 1071 WP_000108937.1 histidinol-phosphate transaminase -
GU332_RS10555 2169676..2170743 + 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T146949 WP_000767829.1 NZ_CP047609:c2165673-2165419 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T146949 NZ_CP062831:c133276-132971 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT146949 WP_001259255.1 NZ_CP047609:c2165921-2165670 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT146949 NZ_CP062831:c133496-133278 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References