Detailed information of TA system TA146925

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 144210..144904
• Replicon: chromosome
• Accession: NZ_CP047609
• Organism: Escherichia coli strain NMBU_ W06E18

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: A0A0D8WHS4
• Locus tag: GU332_RS00630
• Protein ID: WP_001521903.1
• Coordinates: 144506..144904 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: GU332_RS00625
• Protein ID: WP_000554758.1
• Coordinates: 144210..144503 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GU332_RS00600	139325..140074	+	750	WP_016233951.1	C40 family peptidase	-
GU332_RS00605	140131..140484	-	354	WP_024166466.1	type II toxin-antitoxin system HicB family antitoxin	-
GU332_RS00610	140563..142302	-	1740	WP_021523009.1	flagellar biosynthesis protein FlhA	-
GU332_RS00615	142247..143032	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
GU332_RS00620	143103..144158	+	1056	WP_001226177.1	DNA polymerase IV	-
GU332_RS00625	144210..144503	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
GU332_RS00630	144506..144904	+	399	WP_001521903.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
GU332_RS00635	144914..145366	+	453	WP_023144376.1	GNAT family N-acetyltransferase	-
GU332_RS00640	145611..145865	+	255	WP_001521905.1	hypothetical protein	-
GU332_RS00645	146025..146165	+	141	WP_166739706.1	peptide chain release factor	-
GU332_RS00650	146222..147679	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
GU332_RS00655	147940..148398	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
GU332_RS00660	148490..149734	+	1245	WP_000189541.1	esterase FrsA	-
-	148994..149074	+	81	NuclAT_11	-	-
-	148994..149074	+	81	NuclAT_11	-	-
-	148994..149074	+	81	NuclAT_11	-	-
-	148994..149074	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15471.88 Da
• Isoelectric Point: 8.0949

>T146925 WP_001521903.1 NZ_CP047609:144506-144904 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T146925 NZ_CP062826:661002-661316 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT146925 WP_000554758.1 NZ_CP047609:144210-144503 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT146925 NZ_CP062826:661313-661729 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: A0A0D8WHS4

Antitoxin

• AlphaFold DB: S1QAE3