Detailed information of TA system TA146891

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 146924..147345
• Replicon: plasmid pTrh52_1
• Accession: NZ_CP047601
• Organism: Escherichia coli O84:H7 strain Trh52

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: GUK18_RS23610
• Protein ID: WP_001302184.1
• Coordinates: 146924..147082 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	147147..147345 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUK18_RS23580	142738..143121	-	384	WP_021572424.1	relaxosome protein TraM	-
GUK18_RS23585	143443..144045	+	603	WP_077695337.1	transglycosylase SLT domain-containing protein	-
GUK18_RS23590	144341..145162	-	822	WP_021572426.1	DUF945 domain-containing protein	-
GUK18_RS23595	145281..145568	-	288	WP_021572427.1	hypothetical protein	-
GUK18_RS23600	145724..146026	-	303	WP_001272235.1	hypothetical protein	-
GUK18_RS23605	146326..146686	+	361	Protein_135	hypothetical protein	-
GUK18_RS23610	146924..147082	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	147147..147345	-	199	NuclAT_0	-	Antitoxin
-	147147..147345	-	199	NuclAT_0	-	Antitoxin
-	147147..147345	-	199	NuclAT_0	-	Antitoxin
-	147147..147345	-	199	NuclAT_0	-	Antitoxin
GUK18_RS23615	147357..148076	-	720	WP_021572429.1	plasmid SOS inhibition protein A	-
GUK18_RS23620	148073..148507	-	435	WP_021572430.1	conjugation system SOS inhibitor PsiB	-
GUK18_RS23625	148562..150520	-	1959	WP_021572431.1	ParB/RepB/Spo0J family partition protein	-
GUK18_RS23630	150584..150817	-	234	WP_000005988.1	DUF905 family protein	-
GUK18_RS23635	150880..151419	-	540	WP_000290811.1	single-stranded DNA-binding protein	-
GUK18_RS23640	151445..151651	-	207	WP_000547969.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	papH / papC / papD / hlyB / iroB / iroC / iroD / iroE / iroN / faeJ / faeI / faeH / faeF / faeF / faeE / faeD / faeC	1..158248	158248

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T146891 WP_001302184.1 NZ_CP047601:c147082-146924 [Escherichia coli O84:H7]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T146891 NZ_CP062821:c911930-911727 [Providencia rettgeri]
ATGACCAAATTTGATTATCTGATGCGTTTAAGAAAATGCACAACAATTGAAACTCTTGAGCGTGTCATTGAAAAAAACAA
GTATGAATTATCTGATGATGAGCTAGAGCTATTCTACTCCGCAGCAGATCACCGCTTAGCTGAACTCACAATGAATAAGC
TTTATGATAAAATCCCAGCTTCTGTTTGGAAGTTTGTTAGATAA

Antitoxin

Nucleotide

• Length: 199 bp

>AT146891 NZ_CP047601:c147345-147147 [Escherichia coli O84:H7]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin