Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 589781..590343 Replicon chromosome
Accession NZ_CP047600
Organism Escherichia coli O84:H7 strain Trh52

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag GUK18_RS02910 Protein ID WP_000605675.1
Coordinates 590065..590343 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag GUK18_RS02905 Protein ID WP_000781370.1
Coordinates 589781..590065 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUK18_RS02890 584962..588009 + 3048 WP_077778764.1 formate dehydrogenase-N subunit alpha -
GUK18_RS02895 588022..588906 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
GUK18_RS02900 588899..589552 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
GUK18_RS02905 589781..590065 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
GUK18_RS02910 590065..590343 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GUK18_RS02915 590529..591539 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
GUK18_RS02920 591673..593370 - 1698 WP_000433462.1 malate dehydrogenase -
GUK18_RS02925 593527..593664 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
GUK18_RS02930 593766..593981 - 216 WP_000495766.1 biofilm-dependent modulation protein -
GUK18_RS02935 594326..594757 + 432 WP_001592020.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T146865 WP_000605675.1 NZ_CP047600:c590343-590065 [Escherichia coli O84:H7]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T146865 NZ_CP062815:c792074-791697 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT146865 WP_000781370.1 NZ_CP047600:c590065-589781 [Escherichia coli O84:H7]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT146865 NZ_CP062815:c792532-792164 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAATTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References