Detailed information of TA system TA146861

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 449814..450185
• Replicon: chromosome
• Accession: NZ_CP047600
• Organism: Escherichia coli O84:H7 strain Trh52

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: -
• Locus tag: GUK18_RS02265
• Protein ID: WP_097769663.1
• Coordinates: 449991..450185 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	449814..449992 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUK18_RS02240	445769..447142	+	1374	WP_000123770.1	ATP-dependent RNA helicase DbpA	-
GUK18_RS02245	447271..448206	-	936	WP_001157378.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
GUK18_RS02250	448258..449493	-	1236	WP_023568506.1	site-specific integrase	-
GUK18_RS02255	449495..449710	-	216	WP_000079604.1	excisionase XisR	-
GUK18_RS02260	449810..449998	-	189	WP_001302840.1	DUF1187 family protein	-
-	449814..449992	+	179	NuclAT_0	-	Antitoxin
-	449814..449992	+	179	NuclAT_0	-	Antitoxin
-	449814..449992	+	179	NuclAT_0	-	Antitoxin
-	449814..449992	+	179	NuclAT_0	-	Antitoxin
GUK18_RS02265	449991..450185	-	195	WP_097769663.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
GUK18_RS02270	450242..451051	-	810	WP_000166317.1	recombination protein RecT	-
GUK18_RS02275	451044..453695	-	2652	WP_097769662.1	exodeoxyribonuclease VIII	-
GUK18_RS02280	453794..454069	-	276	WP_021555295.1	phage protein	-
GUK18_RS02285	454143..454319	-	177	WP_000245534.1	hypothetical protein	-
GUK18_RS02290	454313..454534	-	222	WP_000560218.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	448258..495855	47597

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 6990.87 Da
• Isoelectric Point: 8.9493

>T146861 WP_097769663.1 NZ_CP047600:c450185-449991 [Escherichia coli O84:H7]
MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESQTGYGGSKKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T146861 NZ_CP062815:194912-195019 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT146861 NZ_CP047600:449814-449992 [Escherichia coli O84:H7]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Antitoxin