Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2678128..2678630 Replicon chromosome
Accession NZ_CP047594
Organism Escherichia coli strain LD27-1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag GUU88_RS13150 Protein ID WP_000767829.1
Coordinates 2678128..2678382 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag GUU88_RS13155 Protein ID WP_001259255.1
Coordinates 2678379..2678630 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUU88_RS13130 2674621..2675979 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
GUU88_RS13135 2675969..2676031 - 63 WP_010723108.1 membrane protein YoeI -
GUU88_RS13140 2676246..2677175 - 930 WP_000803366.1 LysR family transcriptional regulator -
GUU88_RS13145 2677221..2678045 - 825 WP_053266098.1 SDR family oxidoreductase -
GUU88_RS13150 2678128..2678382 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
GUU88_RS13155 2678379..2678630 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
GUU88_RS13160 2678913..2678963 + 51 WP_001364200.1 his operon leader peptide -
GUU88_RS13165 2679109..2680008 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
GUU88_RS13170 2680014..2681318 + 1305 WP_053266100.1 histidinol dehydrogenase -
GUU88_RS13175 2681315..2682385 + 1071 WP_053266101.1 histidinol-phosphate transaminase -
GUU88_RS13180 2682385..2683452 + 1068 WP_001592220.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T146836 WP_000767829.1 NZ_CP047594:c2678382-2678128 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T146836 NZ_CP062811:223501-223608 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAACGGAAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT146836 WP_001259255.1 NZ_CP047594:c2678630-2678379 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT146836 NZ_CP062811:c223452-223389 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References