Detailed information of TA system TA146821

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2004042..2004413
• Replicon: chromosome
• Accession: NZ_CP047594
• Organism: Escherichia coli strain LD27-1

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: GUU88_RS09795
• Protein ID: WP_001317028.1
• Coordinates: 2004219..2004413 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2004042..2004220 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUU88_RS09770	2000033..2001406	+	1374	WP_000123737.1	ATP-dependent RNA helicase DbpA	-
GUU88_RS09775	2001499..2002434	-	936	WP_001157406.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
GUU88_RS09780	2002486..2003721	-	1236	WP_000040852.1	site-specific integrase	-
GUU88_RS09785	2003723..2003938	-	216	WP_000079604.1	excisionase XisR	-
GUU88_RS09790	2004017..2004226	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	2004042..2004220	+	179	NuclAT_0	-	Antitoxin
-	2004042..2004220	+	179	NuclAT_0	-	Antitoxin
-	2004042..2004220	+	179	NuclAT_0	-	Antitoxin
-	2004042..2004220	+	179	NuclAT_0	-	Antitoxin
GUU88_RS09795	2004219..2004413	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
GUU88_RS09800	2004470..2005279	-	810	WP_000166319.1	recombination protein RecT	-
GUU88_RS09805	2005272..2007296	-	2025	Protein_1908	exodeoxyribonuclease VIII	-
GUU88_RS09810	2007259..2008083	+	825	WP_071788686.1	DUF1376 domain-containing protein	-
GUU88_RS09815	2008090..2008836	+	747	WP_000788970.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1995263..2030235	34972

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T146821 WP_001317028.1 NZ_CP047594:c2004413-2004219 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T146821 NZ_CP062792:c4608315-4607782 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT146821 NZ_CP047594:2004042-2004220 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin