Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 857224..857918 Replicon chromosome
Accession NZ_CP047594
Organism Escherichia coli strain LD27-1

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag GUU88_RS04115 Protein ID WP_001263489.1
Coordinates 857520..857918 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag GUU88_RS04110 Protein ID WP_000554758.1
Coordinates 857224..857517 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUU88_RS04090 852856..853353 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
GUU88_RS04095 853577..855289 - 1713 Protein_795 flagellar biosynthesis protein FlhA -
GUU88_RS04100 855261..856046 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
GUU88_RS04105 856117..857172 + 1056 WP_001226164.1 DNA polymerase IV -
GUU88_RS04110 857224..857517 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
GUU88_RS04115 857520..857918 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
GUU88_RS04120 857928..858380 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
GUU88_RS04125 858698..858904 + 207 Protein_801 RtcB family protein -
GUU88_RS04130 858900..859421 + 522 Protein_802 peptide chain release factor H -
GUU88_RS04135 859478..860935 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
GUU88_RS04140 861196..861654 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 862250..862330 + 81 NuclAT_13 - -
- 862250..862330 + 81 NuclAT_13 - -
- 862250..862330 + 81 NuclAT_13 - -
- 862250..862330 + 81 NuclAT_13 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T146819 WP_001263489.1 NZ_CP047594:857520-857918 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T146819 NZ_CP062792:c3818532-3818215 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT146819 WP_000554758.1 NZ_CP047594:857224-857517 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT146819 NZ_CP062792:c3818222-3817935 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References