Detailed information of TA system TA146802

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 1764176..1764690
• Replicon: chromosome
• Accession: NZ_CP047584
• Organism: Limosilactobacillus fermentum strain AGR1485

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A6C1EMT8
• Locus tag: GJA14_RS08945
• Protein ID: WP_035437199.1
• Coordinates: 1764176..1764439 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: GJA14_RS08950
• Protein ID: WP_118033529.1
• Coordinates: 1764436..1764690 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJA14_RS08930	1759652..1760434	+	783	WP_100184711.1	dimethylargininase	-
GJA14_RS08935	1760822..1762219	+	1398	WP_100184712.1	sodium:proton antiporter	-
GJA14_RS08940	1762645..1763766	+	1122	WP_080662969.1	PTS sugar transporter subunit IIC	-
GJA14_RS08945	1764176..1764439	-	264	WP_035437199.1	Txe/YoeB family addiction module toxin	Toxin
GJA14_RS08950	1764436..1764690	-	255	WP_118033529.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GJA14_RS08955	1764860..1765558	+	699	WP_015639439.1	helix-turn-helix domain-containing protein	-
GJA14_RS08960	1765495..1766403	+	909	WP_137876789.1	IS3 family transposase	-
GJA14_RS08965	1766810..1768168	+	1359	WP_100184421.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (25-78)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-55)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10252.94 Da
• Isoelectric Point: 10.3349

>T146802 WP_035437199.1 NZ_CP047584:c1764439-1764176 [Limosilactobacillus fermentum]
MSYAIKLKRSANKDLKKIKGSYLEKSFLQIIEQLRKDPLAPNQGFEKLVLPIKGFYSRRINIQHRLVYKVDQDTQTVIIY
SAWSHDE

Nucleotide

• Length: 264 bp

>T146802 NZ_CP062783:2066-2341 [Escherichia coli O157:H7]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9672.98 Da
• Isoelectric Point: 4.5141

>AT146802 WP_118033529.1 NZ_CP047584:c1764690-1764436 [Limosilactobacillus fermentum]
MDQIVTPTQARKDLFKIIKRINENNEPVVIKPTKADEKSVVVIGEDDWKAIQETLFLVNHGVDKQIKERGNEPLDDFDDV
WGQL

Nucleotide

• Length: 255 bp

>AT146802 NZ_CP062783:1782-2066 [Escherichia coli O157:H7]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A6C1EMT8