Detailed information of TA system TA146796

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 90013..90277
• Replicon: plasmid p94EC-2
• Accession: NZ_CP047578
• Organism: Escherichia coli strain 94EC

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: GUJ28_RS24570
• Protein ID: WP_001387489.1
• Coordinates: 90125..90277 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	90013..90073 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUJ28_RS24545	85459..85809	-	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
GUJ28_RS24550	85806..86231	-	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
GUJ28_RS24555	86316..87185	-	870	Protein_105	IncI1-type conjugal transfer protein TrbB	-
GUJ28_RS24560	87204..88412	-	1209	WP_001383960.1	IncI1-type conjugal transfer protein TrbA	-
GUJ28_RS24565	88719..89810	-	1092	WP_000426061.1	hypothetical protein	-
-	90013..90073	-	61	NuclAT_0	-	Antitoxin
-	90013..90073	-	61	NuclAT_0	-	Antitoxin
-	90013..90073	-	61	NuclAT_0	-	Antitoxin
-	90013..90073	-	61	NuclAT_0	-	Antitoxin
GUJ28_RS24570	90125..90277	+	153	WP_001387489.1	Hok/Gef family protein	Toxin
GUJ28_RS24575	90349..90600	-	252	WP_001291964.1	hypothetical protein	-
GUJ28_RS24580	90900..91196	+	297	WP_011264046.1	hypothetical protein	-
GUJ28_RS25635	91261..91437	-	177	WP_001054897.1	hypothetical protein	-
GUJ28_RS24585	91829..92038	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
GUJ28_RS24590	92110..92772	-	663	WP_000644796.1	plasmid IncI1-type surface exclusion protein ExcA	-
GUJ28_RS24595	92843..95011	-	2169	WP_058649949.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / aac(3)-IVa / aph(4)-Ia / tet(X4) / floR / fosA3 / blaCTX-M-65	-	1..134426	134426

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T146796 WP_001387489.1 NZ_CP047578:90125-90277 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T146796 NZ_CP062782:c4593241-4592843 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT146796 NZ_CP047578:c90073-90013 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin