Detailed information of TA system TA146785

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 47378..47648
• Replicon: plasmid p94EC-1
• Accession: NZ_CP047577
• Organism: Escherichia coli strain 94EC

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: GUJ28_RS23350
• Protein ID: WP_001312861.1
• Coordinates: 47490..47648 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	47378..47441 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUJ28_RS23305	42440..43003	+	564	WP_001311069.1	class I SAM-dependent methyltransferase	-
GUJ28_RS23310	43155..43403	+	249	WP_002433376.1	hypothetical protein	-
GUJ28_RS23320	43876..44403	+	528	WP_000290793.1	single-stranded DNA-binding protein	-
GUJ28_RS23325	44459..44692	+	234	WP_000006004.1	DUF905 domain-containing protein	-
GUJ28_RS23330	44751..46005	+	1255	Protein_45	ParB/RepB/Spo0J family partition protein	-
GUJ28_RS23335	46060..46494	+	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
GUJ28_RS23340	46491..47210	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
GUJ28_RS23345	47222..47410	-	189	WP_001299721.1	hypothetical protein	-
-	47222..47446	+	225	NuclAT_0	-	-
-	47222..47446	+	225	NuclAT_0	-	-
-	47222..47446	+	225	NuclAT_0	-	-
-	47222..47446	+	225	NuclAT_0	-	-
-	47378..47441	-	64	-	-	Antitoxin
GUJ28_RS23350	47490..47648	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
GUJ28_RS25600	48339..48545	+	207	WP_000547939.1	hypothetical protein	-
GUJ28_RS23360	48570..48857	+	288	WP_000107535.1	hypothetical protein	-
GUJ28_RS23365	48978..49799	+	822	WP_001234469.1	DUF945 domain-containing protein	-
GUJ28_RS23370	50096..50698	-	603	WP_000243709.1	transglycosylase SLT domain-containing protein	-
GUJ28_RS23375	51019..51402	+	384	WP_001151524.1	relaxosome protein TraM	-
GUJ28_RS23380	51589..52278	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / sitABCD	iroN / iroE / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA	1..178137	178137

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T146785 WP_001312861.1 NZ_CP047577:47490-47648 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T146785 NZ_CP062782:1093280-1093615 [Escherichia coli O157:H7]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT146785 NZ_CP047577:c47441-47378 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin