Detailed information of TA system TA146776

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2627797..2628399
• Replicon: chromosome
• Accession: NZ_CP047576
• Organism: Escherichia coli strain 94EC

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: GUJ28_RS12530
• Protein ID: WP_000897305.1
• Coordinates: 2628088..2628399 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GUJ28_RS12525
• Protein ID: WP_000356397.1
• Coordinates: 2627797..2628087 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUJ28_RS12500	2623742..2624644	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
GUJ28_RS12505	2624641..2625276	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
GUJ28_RS12510	2625273..2626202	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
GUJ28_RS12515	2626532..2626774	-	243	WP_001086388.1	hypothetical protein	-
GUJ28_RS12520	2626993..2627211	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
GUJ28_RS12525	2627797..2628087	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
GUJ28_RS12530	2628088..2628399	-	312	WP_000897305.1	hypothetical protein	Toxin
GUJ28_RS12535	2628628..2629536	+	909	WP_001162704.1	alpha/beta hydrolase	-
GUJ28_RS12540	2629600..2630541	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
GUJ28_RS12545	2630586..2631023	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
GUJ28_RS12550	2631020..2631892	-	873	WP_000920762.1	virulence factor BrkB family protein	-
GUJ28_RS12555	2631886..2632485	-	600	WP_001295269.1	glucose-1-phosphatase	-
GUJ28_RS12560	2632584..2633369	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T146776 WP_000897305.1 NZ_CP047576:c2628399-2628088 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T146776 NZ_CP062781:74035-74193 [Escherichia coli O157:H7]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATAGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT146776 WP_000356397.1 NZ_CP047576:c2628087-2627797 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT146776 NZ_CP062781:73772-73970 [Escherichia coli O157:H7]
TCACACGGATTGCCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAATGTGGTCAGCGTGCTGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7