Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1751317..1751858 Replicon chromosome
Accession NZ_CP047576
Organism Escherichia coli strain 94EC

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag GUJ28_RS08500 Protein ID WP_000615982.1
Coordinates 1751580..1751858 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag GUJ28_RS08495 Protein ID WP_000729704.1
Coordinates 1751317..1751577 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUJ28_RS08470 1747100..1747885 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
GUJ28_RS08475 1747857..1749569 + 1713 Protein_1662 flagellar biosynthesis protein FlhA -
GUJ28_RS08480 1749674..1749952 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
GUJ28_RS08485 1749945..1750301 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
GUJ28_RS08490 1750358..1751131 - 774 WP_000543899.1 C40 family peptidase -
GUJ28_RS08495 1751317..1751577 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
GUJ28_RS08500 1751580..1751858 + 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
GUJ28_RS08505 1752014..1752754 + 741 WP_001225679.1 murein L,D-transpeptidase -
GUJ28_RS08510 1752725..1753492 - 768 WP_000333380.1 class II glutamine amidotransferase -
GUJ28_RS08515 1753698..1754276 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T146770 WP_000615982.1 NZ_CP047576:1751580-1751858 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T146770 NZ_CP062780:c4706780-4706466 [Escherichia coli O157:H7]
ATGCAATTTACGGTATACCGCAGTCGCAGTAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT146770 WP_000729704.1 NZ_CP047576:1751317-1751577 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT146770 NZ_CP062780:c4707016-4706783 [Escherichia coli O157:H7]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References