Detailed information of TA system TA146752

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 83232..83496
• Replicon: plasmid p2EC1-1
• Accession: NZ_CP047572
• Organism: Escherichia coli strain 2EC1

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: GTQ78_RS22465
• Protein ID: WP_001387489.1
• Coordinates: 83344..83496 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	83232..83292 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTQ78_RS22450	79334..80404	-	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-
GTQ78_RS22455	80423..81631	-	1209	WP_001383960.1	IncI1-type conjugal transfer protein TrbA	-
GTQ78_RS22460	81938..83029	-	1092	WP_000426061.1	hypothetical protein	-
-	83232..83292	-	61	NuclAT_0	-	Antitoxin
-	83232..83292	-	61	NuclAT_0	-	Antitoxin
-	83232..83292	-	61	NuclAT_0	-	Antitoxin
-	83232..83292	-	61	NuclAT_0	-	Antitoxin
GTQ78_RS22465	83344..83496	+	153	WP_001387489.1	Hok/Gef family protein	Toxin
GTQ78_RS22470	83568..83819	-	252	WP_001291964.1	hypothetical protein	-
GTQ78_RS22475	84119..84415	+	297	WP_011264046.1	hypothetical protein	-
GTQ78_RS23750	84480..84656	-	177	WP_001054897.1	hypothetical protein	-
GTQ78_RS22480	85048..85257	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
GTQ78_RS22485	85329..85991	-	663	WP_000644796.1	plasmid IncI1-type surface exclusion protein ExcA	-
GTQ78_RS22490	86062..88230	-	2169	WP_058649949.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / aac(3)-IVa / aph(4)-Ia / tet(X4) / floR / blaCTX-M-65	-	1..127645	127645

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T146752 WP_001387489.1 NZ_CP047572:83344-83496 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T146752 NZ_CP062780:c457211-456726 [Escherichia coli O157:H7]
ATGGGAATAACGGCTCCTACTCCCCTAACGTCTGAACATAATCTGGCTGACTTTTGCTGTTCCGATCACGGGATGAATGA
ATGGCTAAAAAAGAAAGCGTTAAAAAATCACAGCTCCGGTCTGTCTCGTGTTTACGTTATCTGTATTGCCAATACCCGCC
AGGTAATCGGCTATTACTGCCTTTCTACAGGCAGTATTCAACGTAATTTGGCCCCTGGAGCCATGCGACGTAATGCTCCC
GAATCGTTGCCTGTTGTCGTATTAGGCAGACTGGCAATTGATCAAGCCTGGGCAGGTAAAGGGTTGGGCGTCGCATTGTT
GAAAGATGCAGTCTATCGCACAATGTCCATAGCTCAACAGGTAGGGGTTCGTGCGCTCATTGTTCATGCCTTAGACGATT
CAGTACGCAATTTCTATCTGAAGTATGCCTTTGTACCTTCACCATTCCAGTCACTTACCTTACTGTATCCCATTACTCTG
GAGTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT146752 NZ_CP047572:c83292-83232 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin