Detailed information of TA system TA146745

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3582980..3583674
• Replicon: chromosome
• Accession: NZ_CP047571
• Organism: Escherichia coli strain 2EC1

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: GTQ78_RS17400
• Protein ID: WP_001263493.1
• Coordinates: 3582980..3583378 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: GTQ78_RS17405
• Protein ID: WP_000554757.1
• Coordinates: 3583381..3583674 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTQ78_RS17370	3577980..3579224	-	1245	WP_000189541.1	esterase FrsA	-
-	3578640..3578720	-	81	NuclAT_11	-	-
-	3578640..3578720	-	81	NuclAT_11	-	-
-	3578640..3578720	-	81	NuclAT_11	-	-
-	3578640..3578720	-	81	NuclAT_11	-	-
GTQ78_RS17375	3579316..3579774	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
GTQ78_RS17380	3580035..3581492	+	1458	WP_001293002.1	cytosol nonspecific dipeptidase	-
GTQ78_RS17385	3581549..3582106	-	558	WP_001353768.1	peptide chain release factor H	-
GTQ78_RS17390	3582018..3582284	-	267	WP_001321003.1	hypothetical protein	-
GTQ78_RS17395	3582518..3582970	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
GTQ78_RS17400	3582980..3583378	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
GTQ78_RS17405	3583381..3583674	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
GTQ78_RS17410	3583726..3584781	-	1056	WP_001226164.1	DNA polymerase IV	-
GTQ78_RS17415	3584852..3585637	-	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
GTQ78_RS17420	3585582..3587321	+	1740	WP_160177617.1	flagellar biosynthesis protein FlhA	-
GTQ78_RS17425	3587545..3588042	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T146745 WP_001263493.1 NZ_CP047571:c3583378-3582980 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T146745 NZ_CP062779:2066-2341 [Escherichia coli O157:H7]
ATGGAACTGAAGTGGAGCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT146745 WP_000554757.1 NZ_CP047571:c3583674-3583381 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT146745 NZ_CP062779:1782-2066 [Escherichia coli O157:H7]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6