Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1737589..1738091 Replicon chromosome
Accession NZ_CP047571
Organism Escherichia coli strain 2EC1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag GTQ78_RS08260 Protein ID WP_000767829.1
Coordinates 1737837..1738091 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag GTQ78_RS08255 Protein ID WP_001259255.1
Coordinates 1737589..1737840 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTQ78_RS08230 1732767..1733834 - 1068 WP_032202492.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
GTQ78_RS08235 1733834..1734904 - 1071 WP_000108947.1 histidinol-phosphate transaminase -
GTQ78_RS08240 1734907..1736205 - 1299 WP_001743908.1 histidinol dehydrogenase -
GTQ78_RS08245 1736211..1737110 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
GTQ78_RS08250 1737256..1737306 - 51 WP_001364200.1 his operon leader peptide -
GTQ78_RS08255 1737589..1737840 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
GTQ78_RS08260 1737837..1738091 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
GTQ78_RS08265 1738174..1738998 + 825 WP_000754763.1 SDR family oxidoreductase -
GTQ78_RS08270 1739044..1739973 + 930 WP_000803351.1 LysR family transcriptional regulator -
GTQ78_RS08275 1740188..1740250 + 63 WP_010723108.1 membrane protein YoeI -
GTQ78_RS08280 1740240..1741598 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
GTQ78_RS08285 1741777..1742835 + 1059 WP_000492340.1 transport protein YeeE -
GTQ78_RS08290 1742849..1743076 + 228 WP_000234896.1 sulfurtransferase TusA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T146732 WP_000767829.1 NZ_CP047571:1737837-1738091 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T146732 NZ_CP062778:3079101-3079208 [Escherichia coli O157:H7]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT146732 WP_001259255.1 NZ_CP047571:1737589-1737840 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT146732 NZ_CP062778:c3079053-3078994 [Escherichia coli O157:H7]
GTCTGGTTTCAAGATTAGCCCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References