Detailed information of TA system TA146713

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 849838..850513
• Replicon: chromosome
• Accession: NZ_CP047570
• Organism: Enterobacter hormaechei strain F2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A837FDC1
• Locus tag: GTQ93_RS04165
• Protein ID: WP_015571638.1
• Coordinates: 850214..850513 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GTQ93_RS04160
• Protein ID: WP_032669343.1
• Coordinates: 849838..850203 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTQ93_RS04140	846294..847025	+	732	WP_017382760.1	methyltransferase domain-containing protein	-
GTQ93_RS04145	847022..847624	-	603	WP_032654728.1	short chain dehydrogenase	-
GTQ93_RS04150	847722..848609	+	888	WP_015571641.1	LysR family transcriptional regulator	-
GTQ93_RS04155	848630..849811	+	1182	WP_032669342.1	PLP-dependent aminotransferase family protein	-
GTQ93_RS04160	849838..850203	-	366	WP_032669343.1	HigA family addiction module antidote protein	Antitoxin
GTQ93_RS04165	850214..850513	-	300	WP_015571638.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GTQ93_RS04170	850764..851924	-	1161	WP_085929677.1	HlyD family efflux transporter periplasmic adaptor subunit	-
GTQ93_RS04175	851944..854136	-	2193	WP_017692801.1	type I secretion system permease/ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	luxS	800329..854136	53807

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (25-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11624.29 Da
• Isoelectric Point: 9.6776

>T146713 WP_015571638.1 NZ_CP047570:c850513-850214 [Enterobacter hormaechei]
MINHFRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SEGKADDLYLSPHKYTQHK

Nucleotide

• Length: 300 bp

>T146713 NZ_CP062775:2066-2341 [Escherichia coli O157:H7]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13706.72 Da
• Isoelectric Point: 8.5370

>AT146713 WP_032669343.1 NZ_CP047570:c850203-849838 [Enterobacter hormaechei]
MTLEQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGKPDVTLHGNSA

Nucleotide

• Length: 366 bp

>AT146713 NZ_CP062775:1782-2066 [Escherichia coli O157:H7]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A837FDC1