Detailed information of TA system TA146694

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1364533..1365135
• Replicon: chromosome
• Accession: NZ_CP047555
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain SJTUF10112

Toxin (Protein)

• Gene name: higB
• Uniprot ID: C0Q3J8
• Locus tag: DXN18_RS06640
• Protein ID: WP_001159630.1
• Coordinates: 1364533..1364844 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DXN18_RS06645
• Protein ID: WP_000362050.1
• Coordinates: 1364845..1365135 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DXN18_RS06605 (1359646)	1359646..1360245	+	600	WP_000965695.1	glucose-1-phosphatase	-
DXN18_RS06610 (1360239)	1360239..1361111	+	873	WP_000921427.1	virulence factor BrkB family protein	-
DXN18_RS06615 (1361108)	1361108..1361545	+	438	WP_000560974.1	D-aminoacyl-tRNA deacylase	-
DXN18_RS06620 (1361590)	1361590..1362531	+	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
DXN18_RS06625 (1362546)	1362546..1362992	-	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
DXN18_RS06630 (1362989)	1362989..1363300	-	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
DXN18_RS06635 (1363386)	1363386..1364315	-	930	WP_001127703.1	alpha/beta hydrolase	-
DXN18_RS06640 (1364533)	1364533..1364844	+	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
DXN18_RS06645 (1364845)	1364845..1365135	+	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
DXN18_RS06650 (1365182)	1365182..1366111	-	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
DXN18_RS06655 (1366108)	1366108..1366743	-	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
DXN18_RS06660 (1366740)	1366740..1367642	-	903	WP_000331361.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12326.27 Da
• Isoelectric Point: 9.4460

>T146694 WP_001159630.1 NZ_CP047555:1364533-1364844 [Salmonella enterica subsp. enterica serovar Typhimurium]
MQFIETELFTEDVKKLLDDDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGARGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T146694 NZ_CP062774:946473-946880 [Escherichia coli O157:H7]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT146694 WP_000362050.1 NZ_CP047555:1364845-1365135 [Salmonella enterica subsp. enterica serovar Typhimurium]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT146694 NZ_CP062774:946226-946492 [Escherichia coli O157:H7]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0R9NS64