Detailed information of TA system TA146660

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4801908..4802662
• Replicon: chromosome
• Accession: NZ_CP047550
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain SJTUF10057

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B5F003
• Locus tag: DXN07_RS23495
• Protein ID: WP_000558166.1
• Coordinates: 4801908..4802219 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DXN07_RS23500
• Protein ID: WP_001259011.1
• Coordinates: 4802216..4802662 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DXN07_RS23465 (4797566)	4797566..4798468	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
DXN07_RS23470 (4798465)	4798465..4799100	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
DXN07_RS23475 (4799097)	4799097..4800026	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
DXN07_RS23480 (4800073)	4800073..4800363	-	291	WP_000362050.1	DNA-binding transcriptional regulator	-
DXN07_RS23485 (4800364)	4800364..4800675	-	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	-
DXN07_RS23490 (4800893)	4800893..4801822	+	930	WP_001127703.1	alpha/beta hydrolase	-
DXN07_RS23495 (4801908)	4801908..4802219	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	Toxin
DXN07_RS23500 (4802216)	4802216..4802662	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
DXN07_RS23505 (4802677)	4802677..4803618	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
DXN07_RS23510 (4803663)	4803663..4804100	-	438	WP_000560974.1	D-aminoacyl-tRNA deacylase	-
DXN07_RS23515 (4804097)	4804097..4804969	-	873	WP_000921427.1	virulence factor BrkB family protein	-
DXN07_RS23520 (4804963)	4804963..4805562	-	600	WP_000965695.1	glucose-1-phosphatase	-
DXN07_RS23525 (4805753)	4805753..4806556	-	804	WP_000059693.1	DeoR/GlpR family DNA-binding transcription regulator	-
DXN07_RS23530 (4806590)	4806590..4807486	-	897	WP_001520529.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12432.40 Da
• Isoelectric Point: 9.5334

>T146660 WP_000558166.1 NZ_CP047550:4801908-4802219 [Salmonella enterica subsp. enterica serovar Typhimurium]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTFTQPEEMKRYIPSLDNFKYRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYYRGNKE

Nucleotide

• Length: 312 bp

>T146660 NZ_CP062771:431608-432582 [Escherichia coli O157:H7]
GTGGGGCGTGCCGGTCGCGGTATCGATTCTGCTGTTGGCATACTGCTTTGCGGCAGTGAAGACCGTGCTATTCATAAATT
CTTCCGTGAAAGCGCCTTTCCTGCGGAGGCGCAAATTCATGAAATACTCAACGTACTTAGCGTGAATGACGGTCTTACCT
TACGAGGCATTGAACAACGGACGAATCTCCGTTATGGGCAAATAGAAAAAGCACTGAAATTATTGGTAGCTGAAAATCCA
TCGCCTGTGGTGTATACCGAGAAATTATGGCGCAGAACAATTGTCAGTTTTTCTCCTGACCATGAACGAATTAACCATTT
GATGAATCAGAGAAAAAGTGAGCTGGCAGACGTTGAAAGCTATATCACGACCAAGGAGTGCAAAATGCAATTTCTGCGCC
GCGCACTCGATGAGCCAGGTGCCGAACATTGTGGTAAATGTAGCAGTTGTCTTCAGCATCCGTTGTTGTCGCCCGACATT
GATAGCGGCTTACTCCATGCGGCAAACTTATTTATTAAACACGCTGACCTGTCATTAAATCTCAATAAACAGGTGGCAGC
TGGGGCTTTTACGCAATACGGATTTAAAGGGAACTTGCCTGCGAGTTTACAAGGATCTACGGGGCGAATCCTTTCTCGTT
GGGGATATTCCGGGTGGGGAAAGCAGGTAGCACAGGAGAAAAAAACGGGGCGATTCAGTGATGAACTGGTGGAGGCATGT
GCGGAAATGGTTCGCCAACGCTGGAATCCGCATCCTGAACCAACCTGGGTATGCTGCGTTCCTTCATTAAAGCACCTCGA
CCTGGTTCCTGATTTTGAAAGCACCTCGACCTGGTTCCTGATTTTGCCCGGCGACTGGCGGCGAAACTTGGCTTACCTTT
TATTGATGCCATTGAAAAAGTCGTGGACAATCCACCGCAGAAAATGCAGCAAAACCGTTTCCACCAGTGTCAAAATCTCG
ACGGGGCGTTTGTGA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16734.08 Da
• Isoelectric Point: 6.6451

>AT146660 WP_001259011.1 NZ_CP047550:4802216-4802662 [Salmonella enterica subsp. enterica serovar Typhimurium]
MRTHRQMDATSAKKIVDTFSDAVKTVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT146660 NZ_CP062771:432641-433936 [Escherichia coli O157:H7]
ATGGACGCTGACAGTTCTAACAGCACTGTTACGCCAGGCAGGTTGCCCGACGGTTTATCCTCTTGCCCTTGCTTCTACCT
CGGTAAAAAATTGATGAATCTTTCAGCCAATGCACAAGCAACTCTCCTGCTAACCAGCGATTTTTCTCGCGCGGCGGCGA
GTAAGTATAAACCTCTTAGTAATAGTGAATGGGGGAAGTTTGCATTATGGCTGAAGCACCAACGTATCAGTCCCGCCGAG
CTTCTGGTGCCGCAACCGCAAGAGAAACTTACAGGCTGGAGCGATCCGCGTATTTCTCAGGAGCGTATTCTTGGCTTGCT
GGCGCGTGGTCATAGTCTGGCGTTGGCGGTAGATAAGTGGCAACGCGCCGGTTTATGGATCTTAACCCGCGGAGATGCTG
ATTATCCCGTTCGCTTGAAAAACCGATTGCGAACGGATGCACCTCCCGTTTTATTTGGCTGCGGGAATAAAGCATTACTG
CAAGCGGAAGGTATGGCGATTGTTGGCTCGCGAGATGCTCCGACTGACGATTTGCGCTATACCCAACAACTGGCCGCGAA
ACTGGCCCAACAGGGGATTTGCGTTATCTCTGGTGGTGCGCGAGGTATTGATGAATGTGCAATGGCGTCGGCACTGGAGG
CCGGGGGAACTGCCGTTGGCGTATTAGCTGATAGCTTGTTAAAAACGAGTACGTTAGTGAAATGGCGTGAAGGGCTTATA
GCAGGCAACCTGGTGTTGATTTCGCCGTTTTACCCAGAGGTACGTTTCACCGTCGGCAATGCGATGGCGCGAAATAAATA
TATTTATTGCCTTGCTGAAAGCGCAATGGTTGTACGTGCGGGAATGACCGGTGGAACGATAACCGGGGCGATGGAGGCAT
TAAAACATCAGTGGCTGCCTGTGCAGGTTAAACCAAATCAGGATATGCAATCAGCCAATTCACGATTAGTAGAAAATGGG
GCGTCATGGAGTGCTGAACAGGCTGAGAATGTGACGATCAGACTGCCAGACGTTCCTGGGCTGATGTATGACAGAGCACT
CCGTAACGCTCAACCAGAACTGTTTTCGCTGCATGAAGATGACGCAAATTACGCAGTAATGCCCGCGTATACGCCTGTCG
ATTTTTATCAACTCTTTGTGGCGGAACTGGCGATCCTTGCAAAGGAATCGATAAGTATTGAAAGGCTGGCGTCTTGTACT
GGTTTAACCATCGAACAAATTAGTGTGTGGCTGAACCGCGCAGAAGAAGAGGGAAGGGTTATCCGATTGGGCGAAGGTCA
TTATCAGTTCAGGTAA

Structures

Toxin

• AlphaFold DB: A0A0R9ME90