Detailed information of TA system TA146612

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 194468..195222
• Replicon: chromosome
• Accession: NZ_CP047548
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain SJTUF10169

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B5F003
• Locus tag: DXN26_RS00970
• Protein ID: WP_000558166.1
• Coordinates: 194911..195222 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DXN26_RS00965
• Protein ID: WP_001259011.1
• Coordinates: 194468..194914 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DXN26_RS00935 (189644)	189644..190540	+	897	WP_001520529.1	sugar kinase	-
DXN26_RS00940 (190574)	190574..191377	+	804	WP_000059693.1	DeoR/GlpR family DNA-binding transcription regulator	-
DXN26_RS00945 (191568)	191568..192167	+	600	WP_000965695.1	glucose-1-phosphatase	-
DXN26_RS00950 (192161)	192161..193033	+	873	WP_000921427.1	virulence factor BrkB family protein	-
DXN26_RS00955 (193030)	193030..193467	+	438	WP_000560974.1	D-aminoacyl-tRNA deacylase	-
DXN26_RS00960 (193512)	193512..194453	+	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
DXN26_RS00965 (194468)	194468..194914	-	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
DXN26_RS00970 (194911)	194911..195222	-	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	Toxin
DXN26_RS00975 (195308)	195308..196237	-	930	WP_001127703.1	alpha/beta hydrolase	-
DXN26_RS00980 (196455)	196455..196766	+	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	-
DXN26_RS00985 (196767)	196767..197057	+	291	WP_000362050.1	DNA-binding transcriptional regulator	-
DXN26_RS00990 (197104)	197104..198033	-	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
DXN26_RS00995 (198030)	198030..198665	-	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
DXN26_RS01000 (198662)	198662..199564	-	903	WP_000331361.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12432.40 Da
• Isoelectric Point: 9.5334

>T146612 WP_000558166.1 NZ_CP047548:c195222-194911 [Salmonella enterica subsp. enterica serovar Typhimurium]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTFTQPEEMKRYIPSLDNFKYRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYYRGNKE

Nucleotide

• Length: 312 bp

>T146612 NZ_CP062766:3106641-3106919 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16734.08 Da
• Isoelectric Point: 6.6451

>AT146612 WP_001259011.1 NZ_CP047548:c194914-194468 [Salmonella enterica subsp. enterica serovar Typhimurium]
MRTHRQMDATSAKKIVDTFSDAVKTVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT146612 NZ_CP062766:3106448-3106639 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A0R9ME90