Detailed information of TA system TA146609

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4797641..4798243
• Replicon: chromosome
• Accession: NZ_CP047546
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain SJTUF10236

Toxin (Protein)

• Gene name: higB
• Uniprot ID: C0Q3J8
• Locus tag: DXN28_RS23485
• Protein ID: WP_001159630.1
• Coordinates: 4797932..4798243 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DXN28_RS23480
• Protein ID: WP_000362050.1
• Coordinates: 4797641..4797931 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DXN28_RS23465 (4795134)	4795134..4796036	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
DXN28_RS23470 (4796033)	4796033..4796668	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
DXN28_RS23475 (4796665)	4796665..4797594	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
DXN28_RS23480 (4797641)	4797641..4797931	-	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
DXN28_RS23485 (4797932)	4797932..4798243	-	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
DXN28_RS23490 (4798461)	4798461..4799390	+	930	WP_001127703.1	alpha/beta hydrolase	-
DXN28_RS23495 (4799476)	4799476..4799787	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
DXN28_RS23500 (4799784)	4799784..4800230	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
DXN28_RS23505 (4800245)	4800245..4801186	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
DXN28_RS23510 (4801231)	4801231..4801668	-	438	WP_131166381.1	D-aminoacyl-tRNA deacylase	-
DXN28_RS23515 (4801665)	4801665..4802537	-	873	WP_000921427.1	virulence factor BrkB family protein	-
DXN28_RS23520 (4802531)	4802531..4803130	-	600	WP_000965695.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12326.27 Da
• Isoelectric Point: 9.4460

>T146609 WP_001159630.1 NZ_CP047546:c4798243-4797932 [Salmonella enterica subsp. enterica serovar Typhimurium]
MQFIETELFTEDVKKLLDDDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGARGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T146609 NZ_CP062766:2358624-2358727 [Escherichia coli O157:H7]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT146609 WP_000362050.1 NZ_CP047546:c4797931-4797641 [Salmonella enterica subsp. enterica serovar Typhimurium]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT146609 NZ_CP062766:c2358765-2358494 [Escherichia coli O157:H7]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A0R9NS64