Detailed information of TA system TA146584

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4831314..4831916
• Replicon: chromosome
• Accession: NZ_CP047544
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain SJTUF10250

Toxin (Protein)

• Gene name: higB
• Uniprot ID: C0Q3J8
• Locus tag: DXN29_RS23675
• Protein ID: WP_001159630.1
• Coordinates: 4831605..4831916 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DXN29_RS23670
• Protein ID: WP_000362050.1
• Coordinates: 4831314..4831604 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DXN29_RS23655 (4828807)	4828807..4829709	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
DXN29_RS23660 (4829706)	4829706..4830341	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
DXN29_RS23665 (4830338)	4830338..4831267	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
DXN29_RS23670 (4831314)	4831314..4831604	-	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
DXN29_RS23675 (4831605)	4831605..4831916	-	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
DXN29_RS23680 (4832134)	4832134..4833063	+	930	WP_001127703.1	alpha/beta hydrolase	-
DXN29_RS23685 (4833149)	4833149..4833460	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
DXN29_RS23690 (4833457)	4833457..4833903	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
DXN29_RS23695 (4833918)	4833918..4834859	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
DXN29_RS23700 (4834904)	4834904..4835341	-	438	WP_000560974.1	D-aminoacyl-tRNA deacylase	-
DXN29_RS23705 (4835338)	4835338..4836210	-	873	WP_000921427.1	virulence factor BrkB family protein	-
DXN29_RS23710 (4836204)	4836204..4836803	-	600	WP_000965695.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12326.27 Da
• Isoelectric Point: 9.4460

>T146584 WP_001159630.1 NZ_CP047544:c4831916-4831605 [Salmonella enterica subsp. enterica serovar Typhimurium]
MQFIETELFTEDVKKLLDDDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGARGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T146584 NZ_CP062763:c3038867-3038764 [Escherichia coli O157:H7]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT146584 WP_000362050.1 NZ_CP047544:c4831604-4831314 [Salmonella enterica subsp. enterica serovar Typhimurium]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT146584 NZ_CP062763:3038726-3038997 [Escherichia coli O157:H7]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A0R9NS64