Detailed information of TA system TA146534

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4775269..4775871
• Replicon: chromosome
• Accession: NZ_CP047540
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain SJTUF10359

Toxin (Protein)

• Gene name: higB
• Uniprot ID: C0Q3J8
• Locus tag: DXN31_RS23360
• Protein ID: WP_001159630.1
• Coordinates: 4775560..4775871 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DXN31_RS23355
• Protein ID: WP_000362050.1
• Coordinates: 4775269..4775559 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DXN31_RS23340 (4772762)	4772762..4773664	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
DXN31_RS23345 (4773661)	4773661..4774296	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
DXN31_RS23350 (4774293)	4774293..4775222	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
DXN31_RS23355 (4775269)	4775269..4775559	-	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
DXN31_RS23360 (4775560)	4775560..4775871	-	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
DXN31_RS23365 (4776089)	4776089..4777018	+	930	WP_001127703.1	alpha/beta hydrolase	-
DXN31_RS23370 (4777104)	4777104..4777415	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
DXN31_RS23375 (4777412)	4777412..4777858	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
DXN31_RS23380 (4777873)	4777873..4778814	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
DXN31_RS23385 (4778859)	4778859..4779296	-	438	WP_000560974.1	D-aminoacyl-tRNA deacylase	-
DXN31_RS23390 (4779293)	4779293..4780165	-	873	WP_000921427.1	virulence factor BrkB family protein	-
DXN31_RS23395 (4780159)	4780159..4780758	-	600	WP_000965695.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12326.27 Da
• Isoelectric Point: 9.4460

>T146534 WP_001159630.1 NZ_CP047540:c4775871-4775560 [Salmonella enterica subsp. enterica serovar Typhimurium]
MQFIETELFTEDVKKLLDDDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGARGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T146534 NZ_CP062758:4815564-4815905 [Escherichia coli O157:H7]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT146534 WP_000362050.1 NZ_CP047540:c4775559-4775269 [Salmonella enterica subsp. enterica serovar Typhimurium]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT146534 NZ_CP062758:c4815569-4815493 [Escherichia coli O157:H7]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0R9NS64