Detailed information of TA system TA146483

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 196452..197054
• Replicon: chromosome
• Accession: NZ_CP047537
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain SJTUF10405

Toxin (Protein)

• Gene name: higB
• Uniprot ID: C0Q3J8
• Locus tag: DXN32_RS00980
• Protein ID: WP_001159630.1
• Coordinates: 196452..196763 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DXN32_RS00985
• Protein ID: WP_000362050.1
• Coordinates: 196764..197054 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DXN32_RS00945 (191565)	191565..192164	+	600	WP_000965695.1	glucose-1-phosphatase	-
DXN32_RS00950 (192158)	192158..193030	+	873	WP_000921427.1	virulence factor BrkB family protein	-
DXN32_RS00955 (193027)	193027..193464	+	438	WP_000560974.1	D-aminoacyl-tRNA deacylase	-
DXN32_RS00960 (193509)	193509..194450	+	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
DXN32_RS00965 (194465)	194465..194911	-	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
DXN32_RS00970 (194908)	194908..195219	-	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
DXN32_RS00975 (195305)	195305..196234	-	930	WP_001127703.1	alpha/beta hydrolase	-
DXN32_RS00980 (196452)	196452..196763	+	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
DXN32_RS00985 (196764)	196764..197054	+	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
DXN32_RS00990 (197101)	197101..198030	-	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
DXN32_RS00995 (198027)	198027..198662	-	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
DXN32_RS01000 (198659)	198659..199561	-	903	WP_000331361.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12326.27 Da
• Isoelectric Point: 9.4460

>T146483 WP_001159630.1 NZ_CP047537:196452-196763 [Salmonella enterica subsp. enterica serovar Typhimurium]
MQFIETELFTEDVKKLLDDDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGARGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T146483 NZ_CP062755:408069-408596 [Escherichia coli O157:H7]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCTTTAGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT146483 WP_000362050.1 NZ_CP047537:196764-197054 [Salmonella enterica subsp. enterica serovar Typhimurium]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT146483 NZ_CP062755:407796-408062 [Escherichia coli O157:H7]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• AlphaFold DB: A0A0R9NS64