Detailed information of TA system TA146479

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4583141..4583743
• Replicon: chromosome
• Accession: NZ_CP047535
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain SJTUF10452

Toxin (Protein)

• Gene name: higB
• Uniprot ID: C0Q3J8
• Locus tag: DXN08_RS22470
• Protein ID: WP_001159630.1
• Coordinates: 4583432..4583743 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DXN08_RS22465
• Protein ID: WP_000362050.1
• Coordinates: 4583141..4583431 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DXN08_RS22450 (4580634)	4580634..4581536	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
DXN08_RS22455 (4581533)	4581533..4582168	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
DXN08_RS22460 (4582165)	4582165..4583094	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
DXN08_RS22465 (4583141)	4583141..4583431	-	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
DXN08_RS22470 (4583432)	4583432..4583743	-	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
DXN08_RS22475 (4583961)	4583961..4584890	+	930	WP_001127703.1	alpha/beta hydrolase	-
DXN08_RS22480 (4584976)	4584976..4585287	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
DXN08_RS22485 (4585284)	4585284..4585730	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
DXN08_RS22490 (4585745)	4585745..4586686	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
DXN08_RS22495 (4586731)	4586731..4587168	-	438	WP_000560974.1	D-aminoacyl-tRNA deacylase	-
DXN08_RS22500 (4587165)	4587165..4588037	-	873	WP_000921427.1	virulence factor BrkB family protein	-
DXN08_RS22505 (4588031)	4588031..4588630	-	600	WP_000965695.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12326.27 Da
• Isoelectric Point: 9.4460

>T146479 WP_001159630.1 NZ_CP047535:c4583743-4583432 [Salmonella enterica subsp. enterica serovar Typhimurium]
MQFIETELFTEDVKKLLDDDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGARGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T146479 NZ_CP062753:71590-71748 [Escherichia coli O157:H7]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATAGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT146479 WP_000362050.1 NZ_CP047535:c4583431-4583141 [Salmonella enterica subsp. enterica serovar Typhimurium]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT146479 NZ_CP062753:71327-71525 [Escherichia coli O157:H7]
TCACACGGATTGCCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAATGTGGTCAGCGTGCTGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Toxin

• AlphaFold DB: A0A0R9NS64