Detailed information of TA system TA146408

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 197921..198523
• Replicon: chromosome
• Accession: NZ_CP047529
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain SJTUF10640

Toxin (Protein)

• Gene name: higB
• Uniprot ID: C0Q3J8
• Locus tag: DXN10_RS00985
• Protein ID: WP_001159630.1
• Coordinates: 197921..198232 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DXN10_RS00990
• Protein ID: WP_000362050.1
• Coordinates: 198233..198523 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DXN10_RS00950 (193034)	193034..193633	+	600	WP_000965695.1	glucose-1-phosphatase	-
DXN10_RS00955 (193627)	193627..194499	+	873	WP_000921427.1	virulence factor BrkB family protein	-
DXN10_RS00960 (194496)	194496..194933	+	438	WP_000560974.1	D-aminoacyl-tRNA deacylase	-
DXN10_RS00965 (194978)	194978..195919	+	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
DXN10_RS00970 (195934)	195934..196380	-	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
DXN10_RS00975 (196377)	196377..196688	-	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
DXN10_RS00980 (196774)	196774..197703	-	930	WP_001127703.1	alpha/beta hydrolase	-
DXN10_RS00985 (197921)	197921..198232	+	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
DXN10_RS00990 (198233)	198233..198523	+	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
DXN10_RS00995 (198570)	198570..199499	-	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
DXN10_RS01000 (199496)	199496..200131	-	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
DXN10_RS01005 (200128)	200128..201030	-	903	WP_000331361.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12326.27 Da
• Isoelectric Point: 9.4460

>T146408 WP_001159630.1 NZ_CP047529:197921-198232 [Salmonella enterica subsp. enterica serovar Typhimurium]
MQFIETELFTEDVKKLLDDDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGARGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T146408 NZ_CP062746:3578440-3578727 [Escherichia coli O157:H7]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT146408 WP_000362050.1 NZ_CP047529:198233-198523 [Salmonella enterica subsp. enterica serovar Typhimurium]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT146408 NZ_CP062746:3578249-3578440 [Escherichia coli O157:H7]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA

Structures

Toxin

• AlphaFold DB: A0A0R9NS64