Detailed information of TA system TA146379

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4806036..4806638
• Replicon: chromosome
• Accession: NZ_CP047525
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain SJTUF11077

Toxin (Protein)

• Gene name: higB
• Uniprot ID: C0Q3J8
• Locus tag: DXN14_RS23565
• Protein ID: WP_001159630.1
• Coordinates: 4806327..4806638 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DXN14_RS23560
• Protein ID: WP_000362050.1
• Coordinates: 4806036..4806326 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DXN14_RS23545 (4803529)	4803529..4804431	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
DXN14_RS23550 (4804428)	4804428..4805063	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
DXN14_RS23555 (4805060)	4805060..4805989	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
DXN14_RS23560 (4806036)	4806036..4806326	-	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
DXN14_RS23565 (4806327)	4806327..4806638	-	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
DXN14_RS23570 (4806856)	4806856..4807785	+	930	WP_001127703.1	alpha/beta hydrolase	-
DXN14_RS23575 (4807871)	4807871..4808182	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
DXN14_RS23580 (4808179)	4808179..4808625	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
DXN14_RS23585 (4808640)	4808640..4809581	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
DXN14_RS23590 (4809626)	4809626..4810063	-	438	WP_000560974.1	D-aminoacyl-tRNA deacylase	-
DXN14_RS23595 (4810060)	4810060..4810932	-	873	WP_000921427.1	virulence factor BrkB family protein	-
DXN14_RS23600 (4810926)	4810926..4811525	-	600	WP_000965695.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12326.27 Da
• Isoelectric Point: 9.4460

>T146379 WP_001159630.1 NZ_CP047525:c4806638-4806327 [Salmonella enterica subsp. enterica serovar Typhimurium]
MQFIETELFTEDVKKLLDDDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGARGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T146379 NZ_CP062744:3582252-3582539 [Escherichia coli O157:H7]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT146379 WP_000362050.1 NZ_CP047525:c4806326-4806036 [Salmonella enterica subsp. enterica serovar Typhimurium]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT146379 NZ_CP062744:3582061-3582252 [Escherichia coli O157:H7]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA

Structures

Toxin

• AlphaFold DB: A0A0R9NS64