Detailed information of TA system TA146318

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1658250..1658907
• Replicon: chromosome
• Accession: NZ_CP047495
• Organism: Pectobacterium brasiliense strain 1692

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GT391_RS07395
• Protein ID: WP_039320151.1
• Coordinates: 1658536..1658907 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A433N287
• Locus tag: GT391_RS07390
• Protein ID: WP_010281167.1
• Coordinates: 1658250..1658549 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GT391_RS07370	1653521..1654492	+	972	WP_010281174.1	nucleoside hydrolase	-
GT391_RS07375	1654492..1655469	+	978	WP_010281173.1	ABC transporter permease	-
GT391_RS07380	1655466..1656374	+	909	WP_010281171.1	ABC transporter permease	-
GT391_RS07385	1656414..1658213	+	1800	WP_010281169.1	ABC transporter ATP-binding protein	-
GT391_RS07390	1658250..1658549	-	300	WP_010281167.1	XRE family transcriptional regulator	Antitoxin
GT391_RS07395	1658536..1658907	-	372	WP_039320151.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GT391_RS07400	1659245..1659943	+	699	WP_005976717.1	D-galactonate utilization transcriptional regulator DgoR	-
GT391_RS07405	1659940..1660836	+	897	WP_010281154.1	2-dehydro-3-deoxygalactonokinase	-
GT391_RS07410	1660820..1661437	+	618	WP_010281152.1	2-dehydro-3-deoxy-6-phosphogalactonate aldolase	-
GT391_RS07415	1661434..1662582	+	1149	WP_010281150.1	galactonate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 14145.07 Da
• Isoelectric Point: 4.7254

>T146318 WP_039320151.1 NZ_CP047495:c1658907-1658536 [Pectobacterium brasiliense]
MAWEIVTRALFDTWFDEQTETVQEEVLAHLVILEEDGPQLGRPQVDQIKGSKYKNMKELRVQVGGHPFRTFFAFDRTRKA
IVLCAGDKKGEDEKLFYERMIKIADAEFASHLISPEVKEDGDA

Nucleotide

• Length: 372 bp

>T146318 NZ_CP062739:c3580546-3580172 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11048.08 Da
• Isoelectric Point: 10.6442

>AT146318 WP_010281167.1 NZ_CP047495:c1658549-1658250 [Pectobacterium brasiliense]
MATLKEMMAKRSPESRARIEARTAEIRQEITLAKLREELNMSQTQLALALGVKQPSIVKMEKVENDPKLSTLKRYVTALG
GKLTLDVTLPDGKRIALTL

Nucleotide

• Length: 300 bp

>AT146318 NZ_CP062739:c3580967-3580593 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Structures

Antitoxin

• AlphaFold DB: A0A433N287