Detailed information of TA system TA146315

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1174395..1175119
• Replicon: chromosome
• Accession: NZ_CP047495
• Organism: Pectobacterium brasiliense strain 1692

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GT391_RS05185
• Protein ID: WP_010286712.1
• Coordinates: 1174808..1175119 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GT391_RS05180
• Protein ID: WP_010286714.1
• Coordinates: 1174395..1174811 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GT391_RS05155	1169931..1170377	-	447	WP_039318986.1	YhbP family protein	-
GT391_RS05160	1170387..1170833	-	447	WP_010286732.1	helix-turn-helix transcriptional regulator	-
GT391_RS05165	1170987..1172243	+	1257	WP_039318989.1	MFS transporter	-
GT391_RS05170	1172285..1173637	+	1353	WP_010286725.1	aspartate aminotransferase family protein	-
GT391_RS05175	1173732..1174190	-	459	WP_010286715.1	hypothetical protein	-
GT391_RS05180	1174395..1174811	-	417	WP_010286714.1	helix-turn-helix domain-containing protein	Antitoxin
GT391_RS05185	1174808..1175119	-	312	WP_010286712.1	type II toxin-antitoxin system HigB family toxin	Toxin
GT391_RS05190	1175278..1175829	-	552	WP_010286709.1	polymer-forming cytoskeletal protein	-
GT391_RS05195	1176303..1177226	+	924	WP_039318992.1	aminoimidazole riboside kinase	-
GT391_RS05200	1177425..1178960	+	1536	WP_010280265.1	carbohydrate porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-134)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12446.39 Da
• Isoelectric Point: 10.0380

>T146315 WP_010286712.1 NZ_CP047495:c1175119-1174808 [Pectobacterium brasiliense]
MHVISRAPFDTATTQFPNQAAALADLYRVIKREMYATPDDMKKRFPSLDRMKYREKWWVIDIGGGHLRVMFFADFERGKI
FIKHIISHVEYDRLTEYYRRNKE

Nucleotide

• Length: 312 bp

>T146315 NZ_CP062739:2859180-2859335 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15419.69 Da
• Isoelectric Point: 4.6492

>AT146315 WP_010286714.1 NZ_CP047495:c1174811-1174395 [Pectobacterium brasiliense]
MMYADAIKAANNLTSIVPFLGGSTSRKDYDDALKLTEYLIESEPDSPLIDMLTAKIDKYENDAPEFVAFNERIKALPAGI
ALLRVLMDQHQLTQSDFEAEIGKKSLVSRILNGERTLTLDHMRALAKRFDIPVSAFVD

Nucleotide

• Length: 417 bp

>AT146315 NZ_CP062739:c2859168-2859110 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA