Detailed information of TA system TA146304

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 315365..315954
• Replicon: chromosome
• Accession: NZ_CP047492
• Organism: Xanthomonas oryzae pv. oryzae strain IXO1104

Toxin (Protein)

• Gene name: graT
• Uniprot ID: G7TIR7
• Locus tag: IXO1104_RS01405
• Protein ID: WP_012443754.1
• Coordinates: 315365..315646 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IXO1104_RS01410
• Protein ID: WP_011260746.1
• Coordinates: 315664..315954 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IXO1104_RS01400 (IXO1104_01380)	313018..315270	+	2253	WP_012443753.1	exodeoxyribonuclease V subunit alpha	-
IXO1104_RS01405 (IXO1104_01385)	315365..315646	+	282	WP_012443754.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IXO1104_RS01410 (IXO1104_01390)	315664..315954	+	291	WP_011260746.1	HigA family addiction module antitoxin	Antitoxin
IXO1104_RS01415 (IXO1104_01395)	316883..318277	+	1395	WP_027703652.1	type III secretion system effector XopQ	-
IXO1104_RS01420	318380..318567	-	188	Protein_278	hypothetical protein	-
IXO1104_RS01425 (IXO1104_01405)	318727..319059	-	333	WP_011260743.1	hypothetical protein	-
IXO1104_RS01430 (IXO1104_01410)	319314..319640	+	327	WP_241846827.1	hypothetical protein	-
IXO1104_RS01435 (IXO1104_01415)	319768..320190	+	423	WP_033013301.1	PAAR domain-containing protein	-
IXO1104_RS01440 (IXO1104_01420)	320193..320669	+	477	WP_011260741.1	DUF4123 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11066.83 Da
• Isoelectric Point: 10.5318

>T146304 WP_012443754.1 NZ_CP047492:315365-315646 [Xanthomonas oryzae pv. oryzae]
VIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T146304 NZ_CP062739:408069-408596 [Escherichia coli O157:H7]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCTTTAGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT146304 WP_011260746.1 NZ_CP047492:315664-315954 [Xanthomonas oryzae pv. oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT146304 NZ_CP062739:407796-408062 [Escherichia coli O157:H7]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• AlphaFold DB: A0A0K0GFW5