Detailed information of TA system TA146299

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 2564448..2564773
• Replicon: chromosome
• Accession: NZ_CP047485
• Organism: Bacillus subtilis strain BJQ0005

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: P54373
• Locus tag: GTW28_RS13290
• Protein ID: WP_004398662.1
• Coordinates: 2564448..2564627 (+)
• Length: 60 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	2564551..2564773 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTW28_RS13280	2563674..2563811	-	138	WP_021480099.1	hypothetical protein	-
GTW28_RS13285	2563853..2564302	-	450	WP_032722171.1	phage portal protein	-
GTW28_RS13290	2564448..2564627	+	180	WP_004398662.1	type I toxin-antitoxin system toxin TxpA	Toxin
-	2564551..2564773	-	223	NuclAT_0	-	Antitoxin
-	2564551..2564773	-	223	NuclAT_0	-	Antitoxin
-	2564551..2564773	-	223	NuclAT_0	-	Antitoxin
-	2564551..2564773	-	223	NuclAT_0	-	Antitoxin
GTW28_RS13295	2565007..2565096	+	90	WP_075058862.1	type I toxin-antitoxin system toxin BsrH	-
GTW28_RS13300	2565350..2565793	-	444	WP_003229930.1	phage tail tube protein	-
GTW28_RS13305	2565796..2567196	-	1401	WP_003229929.1	phage tail sheath family protein	-
GTW28_RS13310	2567197..2567388	-	192	WP_010886574.1	hypothetical protein	-
GTW28_RS13315	2567385..2567822	-	438	WP_003229927.1	hypothetical protein	-
GTW28_RS13320	2567835..2568338	-	504	WP_003246050.1	HK97 gp10 family phage protein	-
GTW28_RS13325	2568335..2568697	-	363	WP_003229925.1	YqbH/XkdH family protein	-
GTW28_RS13330	2568694..2569089	-	396	WP_004398566.1	DUF3199 family protein	-
GTW28_RS13335	2569093..2569404	-	312	WP_003229923.1	YqbF domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2533871..2587962	54091

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6739.19 Da
• Isoelectric Point: 8.0370

>T146299 WP_004398662.1 NZ_CP047485:2564448-2564627 [Bacillus subtilis]
MSTYESLMVMIGFANLIGGIMTWVISLLTLLFMLRKKDTHPIYITVKEKCLHEDPPIKG

Nucleotide

• Length: 180 bp

>T146299 NZ_CP062737:53972-54277 [Escherichia coli O157:H7]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Nucleotide

• Length: 223 bp

>AT146299 NZ_CP047485:c2564773-2564551 [Bacillus subtilis]
CAAGCAAAAGTATTGCAACTACTGGCGTAAGCTTATAGTTGGTACCACATTACCACATTACCACTTGTTAATGTGTGTTA
CTTTCATGAGAAATGCTAAAATATAAAAGCCAGAGTGTGGCAGCACTCTAGCTTTTAAAAAAGAAACTACCCTTTAATAG
GAGGGTCCTCGTGTAGACACTTTTCCTTTACAGTAATGTAAATAGGATGAGTGTCTTTTTTTC

Structures

Toxin

• AlphaFold DB: A0A164X333

Antitoxin